睾酮对人单核细胞雄激素受体蛋白表达的影响  

Effect of testosterone on expression of androgen receptor in human monocytic cell line THP-1

作　　者：刘长青[1] 吴赛珠[1] 王子东 赖文岩[1] 孙飞[1] 

机构地区：[1]第一军医大学南方医院心内科,广东广州510515 [2]解放军第533医院内一科,云南昆明650000

出　　处：《第一军医大学学报》2004年第4期389-391,共3页Journal of First Military Medical University

基　　金：国家重点研究发展规划("973"课题)(G200057008)~~

摘　　要：目的研究不同浓度的睾酮对人单核细胞中雄激素受体(AR)蛋白表达的影响。方法对培养的人单核细胞株THP-1予不同浓度的睾酮刺激,采用Western blotting检测细胞中AR蛋白表达量,进行半定量比较。结果人的单核细胞中存在AR。AR表达量随睾酮浓度的增加而减少。加用选择性受体阻滞剂氟他胺可使这种作用完全阻滞。结论AR表达量随睾酮浓度的增加而减少,并具有一定的时间、剂量依赖性。Objective To investigate the effect of testosterone on the expression of androgen receptor (AR) in human monocytic cell line THP-1. Methods Cultured THP-1 cells were stimulated by testosterone with different concentrations and the AR protein was examined by Western blotting. Results AR expression was observed in unstimulated THP-1 cells and the quantity of AR expression was reduced by testosterone treatment in a dose-dependent manner. A selective AR blocker com- pletely abolished this inhibitory effect of testosterone on AR expresison. Conclusion Testosterone simulation time- and dose- dependently reduces AR expression in THP-1 cells.

关 键 词：睾酮 人单核细胞雄激素受体 蛋白表达 氟他氨 老年性疾病 

分 类 号：R588[医药卫生—内分泌]