昆明胚鼠生殖腺发育的形态学观察  

Morphological studies on embryonic gonad development in Kunming mice

作　　者：刘小蓉[1] 安靓[1] 李进[1] 

机构地区：[1]第一军医大学组织胚胎学教研室,广东广州510515

出　　处：《第一军医大学学报》2004年第4期415-418,共4页Journal of First Military Medical University

基　　金：广州市重点基金(20002-2-024-01-2)~~

摘　　要：目的研究及观察发育不同时期胚鼠生殖腺发育的形态变化.方法取发育不同时期的胚鼠进行石蜡切片及H、E染色.结果从胚胎切片标本中可观察到,交配后第11天出现生殖嵴,第12天出现未分化生殖腺,第13天可在胚胎标本切片上区分雌雄性,第14天雄性的生殖腺演变为睾丸,出现生精小管,为实心的小管.第16天出现睾丸间质细胞,支持细胞的细胞核明显可见.第16天雌性的生殖腺演变为卵巢,皮质及髓质两者分化明显.第20天睾丸内可见生精小管横断面数目明显增多,管腔内出现空腔,管内有一层精原细胞和一层精母细胞.卵巢内可见一团团粗大的生卵泡性索沿着卵巢周边分布,内含许多原始的卵原细胞,此时有原始的卵泡出现.结论11～12 d为分离原始生殖干细胞(PGCs)最佳时期.Objective To observe the morphological changes of embryonic gonad in Kunming mice in different developmental stages. Methods HE stained paraffin sections of the embryonic mouse gonads in different developmental stages were prepared for evaluation of the morphological changes. Results The genital ridge was identified on the 11th day after the mating and undifferentiated gonad detected on the 12th day, while 1 d later, sexual identification was possible by examining the sections. The male gonad developed into testis by forming seminiferous tubules and Sertoli cells on the 14th day. On the 16th day, the female gonad evolved into the ovary with obvious differentiation of the cortex and medulla and till the 20th day, more seminiferous tubules occurred in the testis with cavities containing numerous spermatogenic cells at different developing phases; also observed was a mass of rough sex cords that produced ovarian follicles surrounding the ovary and contained large quantity of primitive oogonia. Conclusion Days 11-12 after mating might be the best time for seperating primordial germ cells from undifferentiated mouse gonad.

关 键 词：胚鼠 生殖腺 发育 形态学观察 皮质 髓质 

分 类 号：Q954.4[生物学—动物学]