骨髓基质干细胞的分离纯化及培养  

Preparation and culture of murine bone marrow stromal stem cdlls

作　　者：阮绪芝[1] 陈霞萍[2] 严世荣[3] 王卫民[2] 

机构地区：[1]郧阳医学院生物学教研室 [2]郧阳医学院附属太和医院生命科学研究所 [3]郧阳医学院生物化学教研室

出　　处：《生物学杂志》2004年第2期25-27,共3页Journal of Biology

摘　　要：目的　建立骨髓基质干细胞(MSCs)良好的分离纯化和培养方法。方法　将小鼠骨髓基质干细胞自股骨中分离,应用贴壁选择法结合细胞克隆挑选法进行分离纯化,应用细胞生长因子(EGF和PDGF-BB)刺激法进行MSCs的体外培养和传代,倒置显微镜下观察分离培养的细胞并照像记录。结果,培养获得了纯化的呈梭形成纤维样细胞的骨髓基质干细胞。在生长因子EGF和PDGF-BB的共同作用下,传代MSCs生长旺盛,形态均一。结论　该方法是简便高效的骨髓基质干细胞的分离纯化和培养方法。A method for isolating and culturing mouse bone marrow_detived mesenchymal stem cells (mMSCs) were established.MSCs were isolated from mouse thighbone marrow and cultured in Dulbecco's modified eagle's medium with low glucose and purified by wall_sticking and colonies collection,and were proliferated through subculturing by adding epidermal growth factor (EGF)and pletelet_derived growth factor(PDGF-BB).Cellular proliferation were observed and recorded under the inverted microscope.Purified MSCs were acquired and became fibroblast_like.EGF and PDGF_BB were effective agents for mMSCs proliferation in vitro.

关 键 词：小鼠 骨髓基质干细胞 细胞培养 

分 类 号：R331.2[医药卫生—人体生理学]