四种心肌克隆钾通道的表达和电生理记录  

Expression and Electrophysiological Record of Four Kinds of Cloned Myocardial Potassium Channels

作　　者：徐林[1] 黄从新[1] 蒋学俊[1] 李晓艳[1] 吴钢[1] 唐哨勇[1] Duffey M Strauss HC Rasmusson RL 王世敏[1] 

机构地区：[1]武汉大学人民医院心血管内科,湖北武汉430060 [2]Department of Physiology and Biophysics

出　　处：《中国心脏起搏与心电生理杂志》2004年第2期108-111,共4页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：国家自然科学基金资助项目 (批准号 :3 0 0 5 0 0 0 4)

摘　　要：为建立一种研究离子通道的有效模型 ,笔者应用分子生物学技术 ,将包含有Kv1.4、Kv1.5、Herg和KvLQT1等几种心肌克隆钾离子 (K+ )通道cDNA的质粒经扩增 ,提取 ,以及纯化后转录为mRNA。经微注射技术分别将它们注射入克隆离子通道表达系统———非洲爪蟾卵母细胞内 ,然后应用膜片钳技术记录上述克隆离子通道电流。结果 :获得了四种克隆通道的电流 时间关系曲线 (I/t)和电流 电压关系曲线 (I/V)。结论 :本实验结果证明所采用的技术稳定可靠 ,并为今后开展克隆离子通道结构和功能的关系以及抗心律失常药物与离子通道相互作用的研究提供了坚实的基础。To create a model for studying ionic channels,four kinds of plasmids containing four sorts of cloned potassium channel cDNA ( Kv1.4, Kv1.5, Herg and KvLQT 1) were integrated,amplified,extracted and purified,then transcripted to mRNA respectively.These mRNA were injected into Xenopus oocytes,then we recorded their respective currents by patch clamp.Results:According to the experimental data,we derive their current-time (I/t) curves and current-voltage(I/V) curves.Conclusion:These techniques we used are dependable and will be helpful for us to study the relationship between the structure and function of cloned ionic channels as well as the mechanisms of interaction between antiarrhythmic drugs and ionic channels.

关 键 词：心肌克隆钾通道 表达 电生理记录 非洲爪蟾卵母细胞 复极化 膜片钳 

分 类 号：R331.38[医药卫生—人体生理学]