拟步甲昆虫基因组DNA提取的比较研究  

Comparative Study on Genome DNA Extraction of Tenebrionidae Insects

作　　者：代金霞[1] 于有志[1] 郑哲民[2] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]陕西师范大学生命科学学院,陕西西安710062

出　　处：《宁夏大学学报（自然科学版）》2004年第1期66-68,共3页Journal of Ningxia University(Natural Science Edition)

基　　金：国家自然科学基金资助项目 (3976 0 0 17) ;宁夏大学青年基金资助项目

摘　　要：对拟步甲科 8种昆虫的新鲜标本及无水乙醇浸泡标本 ,分别用SDS 蛋白酶K消化法和乙酸钾抽提法进行基因组总DNA的提取 .经 5次重复实验 ,结果表明 ,用两种方法均能从新鲜标本中获得较完整的DNA ,且DNA得率较高 ;而对于无水乙醇保存的标本 ,提取出的DNA用琼脂糖凝胶电泳检测不出DNA条带 ,说明标本在保存过程中DNA可能发生了变化 ,致使提取出的DNA量太少或未分离出DNA .通过对比实验 ,分析原因 ,阐明了两种方法的优缺点 ,并提出了该类昆虫用于DNA提取的标本的保存方法 .This paper reports the extraction genome DNA from eight species of Tenebrionidae insects including the fresh specimens and the preserved specimens in 100 percent alcohol with methods of SDS-proteinase K and KAC. After repeating 5 times, the result shows that the genome DNA can be extracted from the fresh specimens by the methods ,but fail to be extracted from the preserved specimens with the same methods through electrophoresis. It is indicated that genome DNA may be decomposed during preservation, which leads to very little DNA extracted and not enough to be examined by electrophoresis . As the conclusion of the paper, some good suggestions are offered about the specimen preservation.

关 键 词：拟步甲昆虫 基因组DNA 提取方法 昆虫分子系统学 SDS-蛋白酶K消化法 乙酸钾抽提法 

分 类 号：Q969.48[生物学—昆虫学] Q78