家蚕蚕卵超氧化物歧化酶初步研究  

Preliminary Study on Superoxide Dismutase in Eggs of Silkworm

作　　者：魏国清[1] 代君君[1] 田恒杰 王为联 

机构地区：[1]安徽农业大学蚕业丝绸系,合肥230036 [2]安徽省灵璧县农业局,灵璧234200

出　　处：《安徽农业大学学报》2004年第2期194-196,共3页Journal of Anhui Agricultural University

基　　金：安徽省自然科学基金(03041406);安徽省高等学校青年教师科研资助计划项目(2003jq116)资助。

摘　　要：用聚丙烯酰胺凝胶电泳研究蚕卵超氧化物歧化酶(SOD)同工酶的初步性质,利用SOD抑制氮蓝四唑(NBT)光还原反应测定了蚕卵SOD酶学活力,并研究了不同抑制剂对蚕卵SOD活性的影响。结果表明,4个供试蚕品种蚕卵SOD同工酶都有1条主带;蚕卵中SOD同工酶有Mn SOD、CuZn SOD2种类型,以Mn SOD为主,CuZn SOD次之;不同品种之间蚕卵SOD酶学活力相近,同工酶主带一致,副带略有差异。The activity of superoxide dismutase in silkworm eggs was determined by means of polyacrylamide gel electrophoresis and NBT light reducing reaction.The results indicated that the SOD isozyme exhibited a main bright superoxide dismutase band and several dark bands in the gel.Its activity could be strongly inhibited by chloroform-ethanol and slightly inhibited by H_2O_2.So it could be considered that the Mn-SOD exhibited the main activity of superoxide dismutase in the silkworm eggs.

关 键 词：蚕卵 超氧化物歧化酶 抑制剂 

分 类 号：S881.24[农业科学—特种经济动物饲养]