氮源对转基因小球藻异养生长及兔防御素表达的影响  

Effect of Nitrogen Source on Heterotrophic Growth of Transgenic Chlorella and Rabbit Defensin Expression

作　　者：韩兴梅[1] 李元广[1] 魏晓东[1] 孙勇如[2] 王义琴[2] 

机构地区：[1]华东理工大学海洋生化工程研究所生物反应器工程国家重点实验室,上海200237 [2]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《过程工程学报》2004年第1期16-21,共6页The Chinese Journal of Process Engineering

基　　金：国家863计划海洋生物技术主题资助项目(编号:2002AA629110)

摘　　要：在250 ml摇瓶中研究了氮源对转兔防御素(NP-1)基因小球藻的异养生长和NP-1表达的影响,结果表明,转NP-1基因小球藻异养培养的最适氮源为硝酸钾和酵母粉,二者的最佳浓度分别为0.9和9 g/L,藻细胞密度达5.11 g/L,是不添加硝酸钾时细胞密度的1.55倍,而NP-1表达量基本不变. 5 L生物反应器分批培养结果表明,转NP-1基因小球藻在含有硝酸钾和酵母粉两种混合氮源的培养基中培养时,硝酸钾被快速消耗而有机氮源充足,藻细胞内的叶绿素和蛋白质含量下降,但NP-1表达量基本不变.The influence of nitrogen sources on the heterotrophic growth of transgenic Chlorella and rabbit defensin (NP-1) expression in a 250 ml flask was investigated. The results showed that KNO3 and yeast extract were the optimal nitrogen sources for the heterotrophic culture of transgenic Chlorella with NP-1 gene. Dry weight cell density of transgenic Chlorella was improved from 3.29 g/L to 5.11 g/L and NP-1 expression capability kept unchanged as the concentration of KNO3 and yeast extract were 0.9 g/L and 9 g/L respectively. Batch culture of transgenic Chlorella with NP-1 gene in a 5 L bioreactor with the optimized medium was conducted and the process characteristics were also analyzed. It was found that KNO3 was consumed quickly and organic nitrogen was enough in the medium, and chlorophyll and protein contents of transgenic Chlorella decreased while NP-1 expression capability kept unchanged.

关 键 词：转基因小球藻 兔防御素 氮源 异养培养 基因表达 摇瓶试验 

分 类 号：TQ464[化学工程—制药化工]