Streamline SP膨胀床纯化溶菌酶  

Single-step Purification of Lysozyme by an Expanded Bed with Streamline SP

作　　者：赵雪雁[1] 佟晓冬[1] 孙彦[1] 

机构地区：[1]天津大学化工学院生物化工系,天津300072

出　　处：《过程工程学报》2004年第2期103-108,共6页The Chinese Journal of Process Engineering

基　　金：国家杰出青年科学基金资助项目(编号:20025617)

摘　　要：研究了阳离子交换吸附剂Streamline SP在膨胀床内的膨胀特性和流体力学性质,考察了Streamline SP对溶菌酶的吸附平衡和动力学特性,并从卵清蛋白溶液中分离纯化了溶菌酶. 结果表明,通过Streamline SP膨胀床一步分离纯化,溶菌酶活性收率达到86.6%,纯化因子达到62.6.This work is concerned with the development of a single-step process for purification of lysozyme from chicken egg white solution by expanded-bed adsorption. The bed expansion behavior and liquid-phase axial dispersion in the expanded bed with Streamline SP in XK16 column were examined. The results indicated that the expanded-bed system was highly efficient for protein purification. Static experiments showed that the cation exchanger gave a high binding capacity for lysozyme and the bound protein could be dissociated by increasing liquid-phase ionic strength. Kinetic studies led to the estimation of the effective pore diffusivity of lysozyme and its dependence on liquid phase ionic strength. Then, the expanded bed was applied to the single-step purification of lysozyme from a chicken egg white solution. As a result, a recovery yield of 86.6% and purification factor as high as 62.6 were reached. The study indicates that the expanded bed with Streamline SP is promising for direct lysozyme isolation and purification from crude feedstocks.

关 键 词：膨胀床 分离纯化 溶菌酶 阳离子交换吸附剂 流化床 

分 类 号：TQ935[化学工程]