转基因黑杨的抗虫性测定与分析  

Determination and Analysis of Insect-resistant Character in Transgenic Poplars NL-80106

作　　者：郭同斌[1] 嵇保中[1] 诸葛强[1] 蒋继宏[2] 白亚蒙[1] 吴薇[2] 黄敏仁[1] 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037 [2]徐州师范大学生物工程中心,南京221116

出　　处：《分子植物育种》2004年第2期187-191,共5页Molecular Plant Breeding

基　　金：国家研究与开发专项(JY03-B-25);江苏省高新技术项目(BG2001313)资助。

摘　　要：对大田移植的转基因杨树的PCR分析表明 :5个转Bt单基因杨树无性系 (FB4 0、FB4 5、FB5 6、FB5 9、FB6 0 )和 4个转双价基因 (Bt和CpTI)杨树无性系 (D9、D18、D2 7、D32 )均检测到了Bt基因特异片段。在检测的 12个转基因杨树无性系中 ,ELISA结果表明 ,转双价基因杨树无性系中以D18的Bt杀虫蛋白表达量最高 ,Bt杀虫蛋白含量达 6 75 4ng/g鲜叶 ,约占植物可溶性蛋白的 0 0 90 % ;在转Bt单基因杨树无性系中以FB5 1表达量最高 ,达 6 796ng/g鲜叶 ,占植物可溶性蛋白的 0 0 36 %。Transgenic poplars NL-80106 with Bt genes planted in the field were detected by PCR. The results showed that there were the special fragments of Bt genes in 5 transgenic clones with single Bt gene (FB40、FB45、FB56、FB59、FB60) and 4 transgenic clones with two insect-resistant (Bt + CpTI)genes (D9、D18、D27、D32). The leaves from 12 transgenic insect-resistant poplars were analyzed by ELISA and showed that the expressing quantity of Bt insecticidal protein in D18 is 6 754 ng /g fresh leaf (0.090% of plant soluble protein), which is the highest in the transgenic clones with two-insect-resistant genes. In the transgenic clones with single Bt gene, the expressing quantity of FB51 is 6 796 ng/g fresh leaf and it amounts to 0.036% of plant soluble protein.

关 键 词：转基因黑杨 抗虫性 BT基因 CPTI基因 转双价基因 

分 类 号：S763.7[农业科学—森林保护学]