双丰系列甜菜品种(系)AFLP亲缘关系及系谱分析  

AFLP Relationship and Genealogy Analysis of Sugar Beet Sumfull Series Varieties (Lines)

作　　者：于歆[1] 徐德昌[1] 崔杰[1] 吴永英[1] 夏红梅[1] 

机构地区：[1]哈尔滨工业大学食品科学与遗传工程研究院,哈尔滨150086

出　　处：《分子植物育种》2004年第2期229-234,共6页Molecular Plant Breeding

基　　金：国家863计划“空间生物信息获取平台研制”项目(2001AA231091)资助。

摘　　要：应用AFLP技术对甜菜双丰系列的 8个品种 (系 )进行AFLP多态性分析 ,并结合系谱分析对其进行综合评价。从 6 4对引物中筛选出 10对多态性和分辨能力均较高的引物组合 ,10对引物共扩增出 12 38条带 ,其中特异性带 18条 ,特异缺失带 18条 ,特异百分率 2 9%。获得总位点数 2 82个 ,其中共有位点数 177个 ,多态位点百分率为 37 2 %。用Philip3 6软件包进行聚类分析 ,将 8个品种 (系 )聚类为3组。本研究获得的特征带和特征缺失带表明 ,AFLP的高特异分辨率在甜菜品种鉴定上的潜力。分子标记分析和系谱分析证明了供试甜菜品种 (系 )遗传基础狭窄。AFLP technique was employed to analyze the genealogical relationship of 8 sugar beet Sumfull varieties (lines) in this research. 10 pair combinations with high polymorphism and high resolution screened from 64 pair primer enzyme combinations was used to identify the AFLP total 1 238 bands, including 18 specific absent bands and 18 specific bands were obtained. Percent of specific bands is 2.9%. Cluster analyses were performed by using Philip software version 3.6, 3 groups were divided in 8 varieties (lines).It is suggested that genetic basis of the tested varieties (lines) be narrow and AFLP technique could be applied for identification of variety purity and genealogical relationship.

关 键 词：甜菜 双丰系列品种 AFLP 亲缘关系 系谱分析 聚类分析 

分 类 号：S566.3[农业科学—作物学]