荧光分光光度法测定生药厚朴中的厚朴酚与和厚朴酚  

Determination of Magnolol and Honokiol in the Herb of Magnolia officinalis by Fluorescence Spectrophotometry

作　　者：庞立业[1] 陈冠华[1] 田益玲[2] 李月秋[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学食品科技学院,河北保定071001

出　　处：《河北大学学报（自然科学版）》2004年第2期159-162,共4页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目 (2 0 2 2 81) ;河北省教育厅自然科学基金资助项目 (2 0 0 0 2 5 7)

摘　　要：建立了同时测定生药厚朴中厚朴酚与和厚朴酚的荧光测定方法 .液液萃取分离可基本消除样品提取液中共存杂质对测定的影响 ,通过选取适当的光谱校正点可扣除厚朴酚与和厚朴酚的光谱干扰和背景干扰 .方法用于生药厚朴中厚朴酚与和厚朴酚的测定 ,5次测定相对标准偏差分别为厚朴酚 3.97%及和厚朴酚 2 .6 7% ,回收率为厚朴酚 10 2 .6 7%及和厚朴酚 10 1.4 3% .A fluorescence analytical method is developed for the determination of magnolol and honokiol in Magnolia officinalis. The influence of coexistent impurities in the extraction solution of sample on the determination may be eliminated by use of liquid-liquid extraction method. The spectral and background interference for the analytical results can be deducted with an employable compensating process if the calibrated wavelength chosen is suitable. The method has been applied to the determination of magnolol and honokiol in Magnolia officinalis. The relative standard deviations of five detections are 3.97% for magnolol 2.67% for honokiol, and recoveries are 102.67% for magnolol 101.43% for honokiol, respectively.

关 键 词：厚朴酚 和厚朴酚 厚朴 荧光分光光度法 

分 类 号：O658[理学—分析化学]