生物絮凝剂产生菌的筛选及其培养条件优化  

Screening of flocculant-producing strains and optimization on its cultural conditions

作　　者：潘素娟[1] 王荣民[1] 达文燕[2] 牛世全[2] 王云普[1] 

机构地区：[1]西北师范大学甘肃省高分子材料重点实验室 [2]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2004年第2期46-48,51,共4页Journal of Northwest Normal University(Natural Science)

基　　金：甘肃省中青年科技基金资助项目(031-A21-006);甘肃省教育厅基金资助项目(031-29)

摘　　要：从淀粉废水、活性污泥及多种土壤中分离得到32株产絮凝剂菌株,经复筛,得到1株具有较高絮凝活性的细菌SⅥ-4.该菌产絮凝剂的最佳条件为:可溶性淀粉2%,蛋白胨1%,KH2PO40 2%,K2HPO40 2%,NaCl0 05%,(NH4)2SO40 05%,MgSO4·7H2O0 05%,pH6 0～8 0,30～34℃,120r/min,摇床培养2～3d.所产絮凝剂对4‰的高岭土悬浊液的絮凝效率最高可达91 6%.32 strains of flocculant producing microorganisms were isolated from starch wastewater, activated sludge and soil,respectively. After rescreening, 1 strain of bacteria SⅥ-4 with high flocculating activity was obtained. The optimum culture conditions were: soluble starch 2%, peptone 1%, KH_2PO_4 0.2%,K_2HPO_4 0.2%,NaCl 0.05%,(NH_4)_2SO_4 0.05%,MgSO_4·7H_2O 0.05%,pH 6.0～8.0, 30～34 ℃, 120r/min, cultivated for 2～3 days in flakes. The strain produced a strong-activity flocculant, and its best flocculating rate to 4‰ kaolin suspension could reach 91.6%.

关 键 词：生物絮凝剂 筛选 培养条件 

分 类 号：Q93-335[生物学—微生物学]