测定人绒毛膜促性腺素(HCG)的酶免疫传感器的研制  

CONSTRUCTION OF SANDWICH ENZYMEIMMUN-OSENSOR FOR DETECTICN OF HUMAN CHORIONICGONADOTROPIN

作　　者：吕鸣祥[1] 张杰[1] 申群英[1] 陈昆明[2] 冯光易 

机构地区：[1]天津大学 [2]天津医学院 [3]天津海洋技术研究所

出　　处：《中国生物医学工程学报》1992年第3期174-179,共6页Chinese Journal of Biomedical Engineering

基　　金：中国科学院长春应用化学研究所电分析开放实验室项目

摘　　要：本文研制了固定HCG抗体的醋酸纤维素生物活性膜。采用夹心法在膜上发生免疫化学反应,将形成夹心结构的膜,安装在氧电极上,构成酶免疫传感器。耦联过氧化物酶的膜,催化分解H_2C_2致使传感器的输出电流提高,表示为溶液O_2含量的增高。通过溶液中O_2含量的变化值ΔC_2,测定HCG的浓度。被测HCG的浓度范围为5～2500mIU/ml。酶免疫传感器与放射免疫测定相比较,两种方法具有高度相关性,相关系数r=0.998,P<0.001。<ABSTRACT> Construction Sanwich enzyme-immunosensor wos constructed for detec- tion of human chorionic gonadotropin(HCG). The procedure consists of 5 steps: 1 modification of the acetylcellulose membrane by glutaraldehyde(GA) and hexamethylene-diamine (HDA ); 2. coupling of the HCG antibody within the modified membrane; 3. combination of the HCG antigen with HCG antibody within the membrane; 4. binding of the catalase-labeled HCG antibody with HCG antigen on the membrane; and 5. placing the membrane with sandwich structure onto an oxygen electrode. The freed catalase in the membrane will catalyze the decomposition of H2O2 into H2O and O2, thereby the cathodic current of the sensor will increase with the incr ement of oxygen content in the solution. HCG titration is deter- mined based on the oxygen concentration. Preliminary experimental results denyonstrated strated a linear relationship between logarithmic HCG concent- rations and the O2 level within a HCG concentration of 5-2500mIU/ml. A within-batch comparison of reading from the enzyme-immunosensor and radio immunoassay showed a good correlation (r = 0 .998, p <0 .001)and insign- ificant difference (t = 1 .94, p>0. 10 ). The immunosensor is proved fe- asible and expected to be used in serum HCG determination of pregnant women.

关 键 词：HCG 酶免疫传感器 夹心法 

分 类 号：R392.6[医药卫生—免疫学]