鹌鹑心室肌细胞电生理特性的研究  

Study of electrophysiological characteristics of ventricular myocytes in quail

作　　者：荆玉红[1] 龚冬梅[1] 白云龙[1] 刘玉梅[1] 杨宝峰[2] 

机构地区：[1]哈尔滨医科大学药理学教研室 [2]黑龙江省生物医药重点实验室-省部共建国家重点实验室培育基地,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2004年第2期102-105,共4页Journal of Harbin Medical University

基　　金：国家自然科学基金资助课题 ( 3 0 2 715 99)

摘　　要：目的　建立鹌鹑单个心室肌细胞的分离方法 ,观察鹌鹑心室肌细胞的电生理特性 ,并探讨不同离子通道在鹌鹑心肌电活动中的作用。方法　采用酶解法分离单个心室肌细胞 ,应用全细胞膜片钳技术记录心室肌细胞动作电位和L型钙电流。结果　鹌鹑单个心室肌细胞的静息电位 (RP)为 ( 6 2 .2 8± 2 .6 4 )mV ,超射值 (OS)为 (5 9.38±3.81 )mV ,5 0 %复极时间 (APD50 )和 90 %复极时间 (APD90 )分别为 (80 .4 0± 1 9.1 0 )ms和 (1 2 1 .95± 38.72 )ms。在实验电压 +1 0mV时 ,L型钙电流的峰值为 ( 1 3.2 7± 3.70 )pA pF(n =4 )。结论　鹌鹑心室肌细胞上存在着L型钙电流 。Objective To establish the method of isolating single ventricular myocytes of quail and to observe the electrophysiological characteristics of the myocytes and to evaluate the functions of different ionic channels.Methods Enzyme digestion method was used to isolate single ventricular myocytes and whole cell patch clamp technique was used to record action potential and L type calcium current.Results The resting potential (RP) of single ventricular myocytes of quail was (-62.28 ± 2.64) mV, over shooting (OS) was (59.38± 3.81 ) mV, 50% of repolarization (APD 50 ) and 90% of repolarization (APD 90 ) were (80.40 ± 19.10) ms and (121.95 ± 38.72) ms, respectively. At the pulse of +10mV, the peak current density of L type calcium current (I Ca L ) was (-13.27±3.70)pA/pF ( n =4). Conclusion There is a calcium current of L type in quails ventricular myocytes which is a critical current in action potential.

关 键 词：鹌鹑 心室肌细胞 电生理 钙电流 高脂血症 动脉粥样硬化 

分 类 号：R540.4[医药卫生—心血管疾病] R-332[医药卫生—内科学]