大鼠骨髓间充质干细胞分化成心肌细胞的实验研究  

Cardiomyocytes derived from rat bone marrow mesenchymal stem cells

作　　者：张宇[1] 金连弘[1] 李冬梅[1] 李呼伦[2] 刘慧雯[1] 张宝东[1] 

机构地区：[1]哈尔滨医科大学组织胚胎学教研室,黑龙江哈尔滨150086 [2]哈尔滨医科大学神经生物学教研室,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2004年第2期126-128,共3页Journal of Harbin Medical University

摘　　要：目的　采用 5 氮杂胞苷 (5 aza)诱导骨髓间充质干细胞分化成心肌细胞。方法　分离培养骨髓间充质干细胞 ,采用密度为 1 .0 73的percoll分离液分离大鼠骨髓间充质干细胞并进行体外培养和连续传代 ,在第 3代细胞以5 氮杂胞苷进行诱导 ,用免疫组化的方法鉴定心肌特异性肌钙蛋白I的表达。结果　诱导前 ,骨髓间充质干细胞形态均一 ,呈多突起扁平形态 ,诱导后细胞形态发生变化 ,细胞之间形成连接 ,排列方向渐趋一致 ,免疫组化显示表达阳性。结论　 5Objective To study 5 azacytidine (5 aza) which could induce differentiating of mesenchymal stem cells(MSCs) into cardiac myocyte.Methods MSCs were isolated from adult rats. The MSCs were purified by culturing, expanding and expanding. The third passage MSCs were treated by demethylating agent 5 azacytidine. The expression of cardiac Troponin I (cTnI) was observed by immunohistochemistry.Results After the MSCs were treated with 5 aza, the shape of cell changed. The connections of the cells were formed. The direction of the cell arraying was similar gradually. Immunohistochemistry showed that the expression of cardiac Troponin was positive. Electron microscope results showed that fusiform cell had apparent myofilament. The central of cell had an oval shap nucleus.Conclusion 5 aza might induce MSCs differentiating into cardiac myocyte.

关 键 词：大鼠 骨髓间充质干细胞 5-氮杂胞苷 5-AZA 心肌细胞 

分 类 号：R542.2[医药卫生—心血管疾病] R-332[医药卫生—内科学]