水体中Cu^(2+)和Cd^(2+)污染对育珠蚌肝脏中3种酶的影响  

EFFECT OF Cu2+ AND Cd2+ ON THREE ENZYMES IN OYSTER LIVER

作　　者：魏炜[1] 刘克武[1] 赵欣平[1] 石安静[1] 喻东[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《应用与环境生物学报》2004年第2期170-173,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金(No.39970577)资助项目~~

摘　　要：选取两种水体中常见的重金属污染离子(Cu2+和Cd2+),按一定的浓度梯度配制成溶液,并以此溶液饲养育珠蚌一定时间后,测定两种离子对育珠蚌肝脏中的酯酶(EST)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性的影响.结果表明,低浓度的重金属离子可以诱导3种酶活性的升高,而高浓度的离子则抑制酶的活性.同工酶电泳检测显示,高浓度的离子导致EST同工酶谱减少一条谱带,低浓度的离子则诱导产生新的SOD同工酶类型,但两种离子均未对POD同工酶谱产生影响.The effects of two heavy metal ions (Gu2+ and Cd2+ ) on the activities of EST, POD , SOD in oyster liver were studied. The results showed that low concentrations (0. 25 mg L-1 and 0. 5 mg L-1) of the ions could improve the activities of the three enzymes, but high concentrations (0.75 mg L-1 and 1 mg L-1 ) could inhibit the activities. Research of the two ions' effect on the three enzymes 'isoenzyme implied that high concentration of ions could make EST-isoenzyme lose one band, but low concentration had no effect. The effect on SOD-isoenzyme was opposite. High concentration made no effect, while low one could induce new kind of SOD-isoenzyme. But the two heavy ions could not affect POD-isoenzyme. These results revealed that both the enzyme activities and the kinds of isoenzymes would obviously change following the alteration of the ion concentration. Fig 6, Ref 10

关 键 词：水体 CU^2+ CD^2+ 污染 育珠蚌 肝脏 同工酶 酯酶 过氧化物酶 超氧化物歧化酶 重金属 

分 类 号：X52[环境科学与工程—环境工程]