变性梯度胶电泳分析不同喂养方式对早产新生儿肠道菌群的影响(英文)  

Study of intestinal microbiota between breast-feeding and formula-feeding premature neonates by PCR-DGGE

作　　者：李秀云 兰和魁[2] 王霞[2] 符薇[3] 严奇[3] 任大明[3] 

机构地区：[1]广东省一七七医院,广东广州510315 [2]第一军医大学附属珠江医院,广东广州510282 [3]复旦大学遗传所,上海200433

出　　处：《中国现代医学杂志》2004年第7期57-61,共5页China Journal of Modern Medicine

摘　　要：目的　采用变性梯度胶电泳方法 (DGGE)研究喂养方式对早产新生儿肠道菌群的影响。方法　收集同期 6对新生儿 1～ 2 1d粪便 ,直接提取细菌总DNA ,扩增 16SrDNAV6～V8区后DGGE分离 ,测序并与EM BL核苷序列数据库进行比较。结果　喂养前肠道菌群类似 ,以梭状芽孢杆菌、链球菌、克雷伯氏菌为主 ,开奶后母乳喂养儿以双歧杆菌为主 ,奶粉喂养儿肠道菌群显示其明显的多态性 ,有双歧杆菌、梭状芽孢杆菌、链球菌、大肠杆菌、克雷伯氏菌、韦荣氏菌、沙雷氏菌以及不经培养细菌。结论　喂养方式对早产新生儿菌群的形成及演替有明显影响 ,PCR -DGGE在多态性 ,动力性 ,菌群的演进变化方面提供了更加准确的数据和补充资料。Objective:To study the difference of intestinal microbiota between breast-feeding and formula-feeding premature neonates. Methods:Total DNAs were extracted from the feces of two group neonates. Bacterial communities in two group infants were examined by PCR of 16S rDNA V6-V8 regions and denaturing gradient gel electrophoresis(DGGE) and by analyzing the sequences of the ribotypes. Results: During the first few days of life the profiles of DGGE were simple, but they became more complex as the bacterial diversity increased with time, especially in formula-feeding infants. Species most closely related to the Bifidobacterium dominated the intestinal microbiota in breast-feeding infants. Bacterial diversity were more marked in formola-feeding infants than breast-feeding.the bacteria identified included members of genera Bifidobacteria, Clostridium, E.coli, Streptococcus, Uncurtured bacteria, Klesiella, Veillonella, Uncultured fecal bacterium, Serratia.Conclusion: Feeding mode is a important factor affecting developmental intestinal microbiota in premature neonates.PCR-DGGE and 16S rDNA sequence analysis resulted in a dynamic description of bacterial ciloniztion in the infant intestinal microbiota and allowed visualization of bacteria that are difficult to cultivate or be detected by other methods.

关 键 词：变性梯度胶电泳 早产新生儿 肠道菌群 喂养方式 

分 类 号：R722.1[医药卫生—儿科]