白细胞介素-13基因多态性对哮喘患儿血清白细胞介素-13及总免疫球蛋白E水平的影响  

Effect of IL-13 gene polymorphism on the levels of serum IL-13 and total-IgE in asthmatic children

作　　者：陈吉庆[1] 孙海平[2] 郭锡熔[3] 陈荣华[3] 

机构地区：[1]南京医科大学第一附属医院儿科,南京210029 [2]淮安市妇幼保健院 [3]南京医科大学儿科研究所

出　　处：《中国实用儿科杂志》2004年第4期209-211,共3页Chinese Journal of Practical Pediatrics

摘　　要：目的　探讨白细胞介素 1 3(IL 1 3)基因多态性对血清IL 1 3及总免疫球蛋白E(IgE)水平的影响 ,以了解IL 1 3基因多态性在儿童哮喘发病机制中可能的作用。 方法　运用限制性内切酶片断长度多态位点法 (RFLPs)检测哮喘患儿及正常对照组儿童IL 1 3Intron3+1 92 3位点C/T基因多态性 ,并运用ELISA法测定其血清IL 1 3及总IgE水平。 结果　哮喘患儿TT、TC基因型频率分布高于正常对照组 (P <0 0 5 ) ,且TT、TC基因型患儿血清IL 1 3、总IgE水平较CC基因型明显升高 (P <0 0 1 )。 结论　IL 1 3基因多态性在儿童哮喘发病机制中可能起重要的作用。Objective To explore the effect of IL-13 gene polymorphism on the levels of serum IL-13 and total-IgE so as to understand the role of IL-13 gene polymorphism in the mechanism of childhood asthma.Methods Restriction fragment length polymorphism site method (RFLPs) was used to detect+1923 site polymorphism of IL-13 gene in Intron3 region and ELISA method was used to detect the levels of serum IL-13 and total-IgE.Results The frequency distribution of TT and TC genetypes was higher than that of CC genetype ( P <0.05);the levels of serum IL-13 and total-IgE of TT and TC gene types were significantly elevated compared with that of CC genetype( P <0.01).Conclusion IL-13 gene polymorphism may play an important role in the mechamism of childhood asthma.

关 键 词：白细胞介素-13 基因多态性 哮喘 患儿 血清 白细胞介素-13 总免疫球蛋白E 

分 类 号：R725.622.5[医药卫生—儿科]