盐生杜氏藻Ugd基因的cDNA克隆及序列分析  

Molecular cloning and sequence analysis of Ugd gene in Dunaliella.Salina

作　　者：李音[1] 乔代蓉[1] 易弋[1] 贺庆华[1] 李良[1] 孙辉[1] 曹毅[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2004年第2期409-412,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：国家863项目(2002AA213021);国家自然科学基金(30270711)

摘　　要：作者对不同物种Ugd基因的同源序列进行相似性分析后设计一对兼并引物,利用RT PCR技术获得一条200bp左右的片段,测序分析显示其同芋头(Colocasiaesculenta)的Ugd基因的编码区有71.7%的相似性.然后再以此片段为模板设计引物,通过RACE技术获得盐生杜氏藻Ugd基因的全长序列.经克隆测序作blastx分析发现其同芋头(Colocasiaesculenta)、大豆(Soybean)、水稻(Oryzasativa)、拟南芥(Arabidopsisthaliana)的Ugd基因有78%到81%的同源性.The degenerate primers were designed based on the homologous gene of Ugd in other organisms.A cDNA fragment of 200bp from D.salina was obtained by RT-PCR technique. After cloning and DNA sequencing, the result indicated that 71.7% of the sequence of the fragment was homologous to the Ugd in Colocasia esculenta.Based on this sequence, a pairs of primers weredesigned. By the RACE technique, the full-length cDNA of Ugd was successfully constructed in D.salina.

关 键 词：同源克隆 兼并引物 Ugd RACE 

分 类 号：Q78[生物学—分子生物学]