Percoll优选对形态正常精子百分率和顶体酶活性的影响  

Effect of Percoll Selection Technique on Normal Morphology Rate and Acrosin Activity of Human Spermatozoa

作　　者：沙艳伟[1] 王洪亮[2] 刘睿智[1] 许宗革[1] 高久春[1] 王忠山[1] 

机构地区：[1]吉林大学基础医学院细胞生物学教研室 [2]吉林大学第一临床医院泌尿外科,吉林长春130021

出　　处：《中华男科学杂志》2004年第4期263-264,268,共3页National Journal of Andrology

基　　金：吉林省科技厅资助项目 ( 2 0 0 3 0 43 3 )

摘　　要：目的 :了解形态正常精子百分率和顶体酶活性在Percoll优选前后的变化。　方法 :30例不育男性精液标本 ,应用计算机精液分析仪进行精子形态分析 ,分光光度比色法检测顶体酶活性。　结果 :Percoll优选后形态正常精子百分率和精子顶体酶活性均明显高于Percoll优选前 (P均 <0 .0 0 1)。　结论 :Percoll法优选精子后形态正常精子百分率和顶体酶活性增加。Objective: To study the effect of Percoll selection technique on normal morphology and acrosin activity of human spermatoza. Methods: The sperm morphology and sperm acrosin activity were analyzed by automated sperm morphology analyzer(ASMA) and spectrocolorimetry. Results: The normal morphology sperm rate and acrosin activity were significantly increased after Percoll selection technique (P< 0.001 ). Conclusion: Percoll selection technique could affect normal morphology sperm ratio and acrosin activity.

关 键 词：男性不育 精子形态 顶体酶 Percoll优选法 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]