用单克隆抗体酶联免疫吸附测定法检测水稻种子上的白叶枯病菌  

The Detection of Xanthomonas campestris pv. oryzae with the McAb—ELISA after Multiplication

作　　者：李清铣[1] 刘斯文[1] 高锦梁[1] 胡广淦[1] 朱华 黄奔立[1] 

机构地区：[1]江苏农学院,扬州225001

出　　处：《中国水稻科学》1992年第2期93-95,共3页Chinese Journal of Rice Science

摘　　要：我们于1988年建立了以水稻白叶枯病菌单克隆抗体为第二抗体的酶联免疫吸附测定法,虽然具有灵敏、精确、简便、快速的优点,但用于检测水稻白叶枯病病种所带的微量细菌,灵敏度仍然不足,存在漏检现象。因而,我们对利用单克隆抗体检测水稻白叶枯病病种的方法作了进一步的改进研究。A double antibodies sandwich ELISA with the monoclonal antibody as the second antibody for the rapid detection of Xanthomonas campestris pv.oryzae in rice seeds was developed. The bacterial from husk of the seed samples were shake-cultured in sterile liquid medium for 48 hat 25℃, and followed by ELISA detection. 126 seed samples from Jiangsu and Zhejiang were tested, 93% of seed samples from in- fected rice were positive.

关 键 词：种子 水稻 白叶枯病 ELISA 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]