洛沙坦和黄芪治疗糖尿病性肾病的相关机制研究  

Therapeutic effects of losartan and astragalus membranaceus on diabetic nephropathy

作　　者：冯烈[1] 冯毅[2] 卢筱华[1] 

机构地区：[1]暨南大学附属第一医院内分泌科,广东广州510630 [2]中山市人民医院内分泌科,广东中山528400

出　　处：《中国病理生理杂志》2004年第4期655-659,共5页Chinese Journal of Pathophysiology

摘　　要：目的 :观察洛沙坦和黄芪对糖尿病大鼠肾脏病变的治疗作用并探讨其作用机制。方法 :用SD大鼠复制链脲佐菌素糖尿病模型 ,12周后观察洛沙坦和黄芪对糖尿病大鼠肌酐清除率 (Ccr)、尿白蛋白排泄率及肾小球平均体积、肾小球毛细血管基底膜平均厚度的影响 ,并观察血、肾脏局部一氧化氮 (NO)、内皮素 - 1(ET - 1)、转化生长因子 - β1(TGF - β1)水平的变化。结果 :洛沙坦和黄芪可降低糖尿病大鼠Ccr、尿蛋白排泄率及肾小球平均体积、肾小球毛细血管基底膜平均厚度 ,并可不同程度降低肾脏局部NO、ET - 1、TGF - β1水平 ,两药合用可增强上述作用。结论 :洛沙坦和黄芪可延缓糖尿病肾脏结构和功能损害的进程 ,且两药合用效果更佳 ,此作用可能与洛沙坦和黄芪降低肾脏局部NO、ET - 1、TGF - β1水平有关。AIM: To observe the protective effects of losartan and astragalus membranace on the kidney of diabetic rats, and to study their possible mechanisms. METHODS: The diabetic rats were induced by a single intraperitoneal injection of streptozotocin. At the end of 12th week,changes in urinary albumin excretion, urinary β 2-MG excretion, Ccr,NO,ET-1 levels in blood, urinary and renal tissue were observed. Serum and urinary TGF-β 1 concentration,average volume of glomeruler,average thickness of glomerular basement membrane were also measured. RESULTS: In the treated diabetic rats, urinary albumin excretion, urinary β 2-MG excretion, Ccr, urinary and renal tissue NO, urinary TGF-β 1, average volume of glomeruler, average thickness of glomerular basement membrane decreased obviously as compared with diabetic untreated rats. These effects were enhanced when losartan was combined with astragalus membranace. CONCLUSION: Losartan or astragalus membranace reversed the injury of renal structure and function in STZ-induced diabetic rats. The protective effects were enhanced when losartan was combined with astragalus membranace. The decrease in NO,ET,TGF-β 1 concentration in renal tissue may be one of mechanisms for this action.

关 键 词：糖尿病肾病 洛沙坦 膜荚黄芪 一氧化氮 内皮缩血管肽Ⅰ 转化生长因子-Β 

分 类 号：R587.1[医药卫生—内分泌]