新生猪磨牙牙根发育cDNA消减文库的建立  

Construction of cDNA Library of the Related Gene during Pig Molar Root Patterning

作　　者：郑梁[1] 肖明振[1] 金岩[1] 轩昆[1] 李鑫[1] 

机构地区：[1]第四军医大学口腔医学院牙体病科,陕西西安710032

出　　处：《口腔医学研究》2004年第2期146-148,共3页Journal of Oral Science Research

基　　金：第四军医大学创新基金重点课题资助项目 (编号 :CXO2F0 0 2 )

摘　　要：目的 :分析新生猪前后牙基因的表达差异并获取新的表达序列。方法 :以新生猪第一恒磨牙牙胚为检验方 ,恒中切牙牙胚为驱动方 ,进行消减杂交 ,经克隆筛选获得新生猪恒磨牙的消减文库。结果 :随机挑选出 6 0个阳性克隆 ,酶切后获得 2 0 0～ 2 0 0 0bp的插入片断。结论Objective: To construct the differentially expressed cDNA library of the related gene subtracted from incisor and molar during pig teeth root patterning. Methods: Total mRNA sample of incisor germ were extracted as tester and that of molar germ were extracted as driver, cDNA library was constructed By modified subtractive hybridization technique. Results: After enzyme digestion there were inserted fragments (200～2 000 bp) in 60 cloning selected randomly. Conclusions: The differentially expressed cDNA library was constructed successfully.

关 键 词：CDNA消减文库 磨牙 牙根发育 差异表达基因 新生猪 

分 类 号：R346[医药卫生—基础医学]