Leptin长型受体与生长抑素共存于猪前脑内  

Coexistence of leptin long form receptor and somatostatin in forebrain of pig

作　　者：樊明欣[1] 雷治海[1] 李庆梅[1] 王红星[1] 

机构地区：[1]南京农业大学动物医学院,南京210095

出　　处：《解剖学杂志》2004年第2期117-120,F003,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金 (39970 5 5 5 )

摘　　要：目的 :研究三元杂交猪和梅山猪仔猪前脑内Leptin长型受体 (Ob Rb)mRNA与生长抑素 (SS)免疫反应阳性物质的共存关系。方法 :用原位杂交与免疫组织化学相结合的方法。结果 :Leptin长型受体mRNA和生长抑素免疫反应阳性神经元分布于下丘脑室周核、背内侧核、腹内侧核、室旁核、海马 杏仁核及大脑皮质。上述结构内一些表达Leptin长型受体mRNA的神经元也含有生长抑素免疫反应阳性物质。 结论 :两种猪前脑内Ob RbmRNA与SS免疫反应阳性物质的共存情况无明显差异。Leptin可直接作用于下丘脑的生长抑素免疫反应阳性神经元调节动物的能量代谢 生长和繁殖等活动。Objective: To investigate the coexistence of the long form leptin receptor(Ob-Rb) mRNA and somatostatin-like immunoreactivity(SS-IR) in neurons of the pig forebrain. Methods: Combination of in situ hybridization and immunohistochemistry was used in this study. Results: (1) Both Ob-Rb mRNA and SS-IR containing neurons were seen in the periventricular, dorsomedial, ventromedial and paraventricular nuclei of the hypothalamus, the hippocampal formation, amygdala and cortex of the pig forebrain. (2) Some of neurons expressing Ob-Rb mRNA also contained SS-IR. Conclusion: There was no obvious difference in coexistence of Ob-Rb mRNA and SS-IR containing neurons between Landrace and Meishan pigs. Leptin may directly regulate food intake, energy metabolism, growth and reproduction through the SS neurons which expressing Ob-Rb in the hypothalamus of the pigs.

关 键 词：Leptin长型受体 生长抑素 猪 前脑 原位杂交法 免疫组织化学 免疫反应阳性神经元 下丘脑 

分 类 号：S852.2[农业科学—基础兽医学]