氯化锂对成年小鼠海马神经发生的影响  

Lithium chlorine promoting neurogenesis in adult mouse hippocampus

作　　者：胡坚莉[1] 张立平[1] 杨允[2] 

机构地区：[1]山东大学医学院解剖学教研室,济南250012 [2]山东省立医院骨科

出　　处：《解剖学杂志》2004年第2期127-130,共4页Chinese Journal of Anatomy

基　　金：山东省自然科学基金 (Y99C0 3)

摘　　要：目的 :观察氯化锂 (LiCl)对成年小鼠海马细胞增殖与神经发生的影响。方法 :用 5 溴 2 脱氧尿核苷 (BrdU)标记新生细胞 ,免疫组化染色后统计新生细胞数量 ;应用BrdU与抗微管蛋白单克隆抗体 (JuJI) /抗胶质纤维酸性蛋白多克隆抗体 (GFAP)双标记 ,鉴别出BrdU阳性细胞的细胞类型。结果 :LiCl组小鼠海马齿状回细胞增殖与神经发生明显强于对照组 ;新增殖的细胞约有 70 %为神经元 ,5 %左右为神经胶质细胞。结论 :LiCl增强了成年小鼠海马齿状回的细胞增殖与神经发生 ,这一功能对于中枢神经系统退行性疾病的预防与治疗可能有重要的意义。Objective: To study the effect of lithium chlorine on cell proliferation and neurogenesis in the adult mouse hippocampus. Methods: Mice were chronically treated with lithium chlorine and proliferating cells were labelled with 5-bromo-2'-deoxyuridine (BrdU) for over 10 d. BrdU immunohistochemistry were conducted to dertermine the number of newly generated cells and double immunolabelling was used to co-localize BrdU-positive cells with TuJ1 (tubulin-βAb-3, neuron-specific early differentiation marker) /GFAP (rabbit anti-glial fibrillary acid protein). Results: Lithium chlorine produced a significant increase in the cell proliferation and neurogenesis in the dentate gyrus. 70% of the cells were double-labelled with BrdU and TuJ1, and 5% double-labelled with BrdU and GFAP. Conclusion: These findings suggest that lithium chlorine significantly increases cell proliferation and neurogenesis in the adult mouse dentate gyrus and may be usful in the treatment of certain neurodegenerative diseases.

关 键 词：氯化锂 成年小鼠 海马 神经发生 单克隆抗体 细胞增殖 中枢神经系统退行性疾病 

分 类 号：R965[医药卫生—药理学]