大鼠下边缘皮质-伏隔核投射神经元胞体受对称性突触支配  

Neurons in rat infralimbic cortex projecting to nucleus accumbens shell are innervated by symmetric synapses

作　　者：李瑞锡 [1] 彭裕文 [1] 崛悦郎  大谷修  西条寿夫  王劼 [1] 丁忠良 [1] 高璐 [1] 

机构地区：[1]复旦大学上海医学院解剖学与组织胚胎学系,上海,200032 [2]日本国富山医科药科大学生理学 [3]日本国富山医科药科大学解剖学

出　　处：《解剖学杂志》2004年第2期131-134,共4页Chinese Journal of Anatomy

基　　金：复旦大学引进人才基金 (EXF 10 2 30 2 )

摘　　要：目的 :揭示向伏隔核壳部 (nucleusaccumbensshell,AcbSh)投射的下边缘皮质 (infralimbiccortex ,IL)神经元胞体上的突触体系 ,增加该神经通路中的突触学资料。方法 :注射HRP到大鼠的AcbSh ;以TMB法显示IL中被HRP逆行标记的神经元 ;光镜及电镜观察IL中标记神经元的形态、分布及胞体部的突触结构。结果 :含有HRP/TMB反应产物的神经元散在分布于IL中。神经元胞体呈三角形或圆锥体形锥体细胞的基本特征 ,直径约为 1 5μm× 2 5 μm。电镜可见神经元胞体仅接收 3～ 5个对称性突触。 结论 :向AcbSh投射的IL神经元通过对称性突触接受抑制性神经信息支配 ,提示在整个摄食行为神经通路中 ,IL支配AcbSh ,而自身又受抑制性控制。Objective: To obtain the synaptic data on the neural pathway for feeding behaviors by revealing the synaptic connections on the projection neurons from infralimbic lobe (IL) to nucleus accumbens shell (AcbSh) in the rats. Methods: HRP was delivered into the rat AcbSh, and the retrogradely transported HRP in the IL were reacted with TMB. The morphology and distribution of the HRP labelled neurons in the IL were observed under light microscopy, and synapses on the perikarya of these labelled neurons were examined with electron microscopy. Results: The retrogradely labelled neurons containing HRP/TMB reaction product were observed throughout the IL. The perikarya of these labelled neurons showed basic features of the pyramidal cell, and measured approximately 15 μm×25 μm. Under electron microscopy, 3-5 synapses, were observed in each profile of the labelled perikaryon in the IL. Conclusion: In the feeding neural pathway, the neurons in the IL innervate the AcbSh, while the neurons in the IL themselves are controlled by inhibitory information through symmetric synapses.

关 键 词：大鼠 下边缘皮质 伏隔核 神经元胞体 对称性突触 摄食行为 逆行追踪 电子显微镜 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]