骨质疏松骨折愈合中软骨细胞凋亡与血管内皮生长因子的表达  

Expression of VEGF and apoptosis of chondrocytes during osteoporotic fracture healing

作　　者：王松[1] 张晛[2] 陈国庆[1] 王凡[3] 李华[3] 李瑞祥[3] 

机构地区：[1]武汉体育学院运动解剖学教研室,武汉430079 [2]解放军总医院骨科 [3]四川大学华西医学中心基础医学与法医学院

出　　处：《解剖学杂志》2004年第2期162-165,共4页Chinese Journal of Anatomy

摘　　要：目的 :探讨骨质疏松骨折愈合过程中血管内皮生长因子 (VEGF)的表达变化及其与软骨细胞凋亡发生的关系。方法 :建立SD大鼠骨质疏松股骨骨折模型 ,分别于骨折后 1、2、3、4w取材进行HE染色及VEGF免疫组化染色 ,并用TUNEL法和琼脂糖凝胶电泳法检测细胞凋亡。结果 :在骨折后第 1w ,间充质细胞未见VEGF表达 ;第 2、3w时软骨细胞VEGF反应呈强阳性 ;第 4w其表达强度开始下降。骨折愈合的不同时期都有软骨细胞凋亡的发生 ,第 3w表现最为明显。结论 :VEGF在骨折的第 2w表达最强 ,到第 3、4w开始下降。软骨细胞的凋亡主要发生在骨折后的第 3w。VEGF可能直接与软骨细胞凋亡发生有关。Objective: To study the expression of VEGF and apoptosis of chondrocytes during the osteoporotic fracture healing. Methods: An experimental model of the osteoporotic fracture healing on rat femurs was used. The expression of VEGF were determined by means of immunohistochemistry and the apoptosis of chondrocytes was detected by TUNELmethods and agarose gel eletrophoresis at the different stages of healing. Results: VEGF was expressed in chondrocytes and osteoblasts at first week after fracture. There were no expression of VEGF in mesenchymal cells. On the second, third week after fracture, VEGF was strongly expressed in chondrocytes. During the fourth week, the expression of VEGF began to decrease. There were apoptosis of chondrocytes during whole fracture healing. Apoptosis of chondrocyte peaked during the third week. Conclusion: The levels of VEGF is different at different periods of the osteoporotic fracture healing. VEGF may take part in forming of blood vessels and participate in inducing the apoptosis.

关 键 词：骨质疏松 骨折愈合 软骨细胞 细胞凋亡 血管内皮生长因子 血管生成 动物模型 

分 类 号：R683[医药卫生—骨科学] R-332[医药卫生—外科学]