人促卵泡生成激素IRMA法与RIA法对比的实验观察  

The Establishment of FSH IRMA and Its Comparison with FSH RIA

作　　者：刘胜乾 徐文 

机构地区：[1]北京市北免东雅生物技术研究所,北京100012

出　　处：《标记免疫分析与临床》2004年第1期37-40,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：IRMA法试剂盒采用两株单克隆抗体 ,一株用于包被作为固相抗体 ,另一株用于标记Na1 2 5I为标记物 ,所用标准品与RIA法相同。结果显示 ,IRMA法试剂盒反应时间短 ,不用加分离剂专门离心 ,同时 ,IRMA试剂盒还具有灵敏度高、特异性强和稳定性好等优点 ;两种方法测值相关系数为 0 .980 0。FSH IRMA was established and studied by comparing with FSH RIA.Two highly affinity monoclonal antibodies(McAb) were employed in the assay, one McAb coated to polystyrene tube, and the other labeled with Na 125 I tracer. Correlation coefficient between IRMA and RIA is 0.9800. FSH IRMA is comparably fast and convenient.Moreover, FSH IRMA has more high sensitivity, specificity and stability when compared with FSH RIA.

关 键 词：人促卵泡生成激素 免疫放射分析法 放射免疫分析法 单克隆抗体 

分 类 号：R817[医药卫生—影像医学与核医学]