猪繁殖——呼吸综合症(PRRS)抗体免疫金标快速检测试纸的研制与应用  

Study and Apply for Detection of PRRS Anti-body by ColloidalGgold Stripe

作　　者：方莹[1] 黄印尧[2] 张长弓[2] 颜江华[1] 

机构地区：[1]厦门大学抗癌中心 [2]厦门出入境检验检疫局,361012

出　　处：《福建畜牧兽医》2004年第2期1-2,共2页Fujian Journal of Animal Husbandry and Veterinary medicine

摘　　要：应用酶联免疫吸附原理和胶体金层析技术,在玻璃纤维和硝酸纤维素膜的检测线和对照线上分别喷上胶体金PRRSV,PRRSV和兔抗PRRS抗体,从而制作成猪PRRS抗体免疫金标试纸,用该试纸与PRRS-ELISA试剂盒分别对30份猪血清及血液样品进行抗体水平检测,结果完全一致。说明金标试纸法是一种微量、特异、简便和结果容易判定的新的检测方法,非常适用于基层兽医站和猪场,特别是进行现场检测和开展大规模疫情普查时使用。Based on the principle of immune - ELISA and colloidal gold immunochromatography, colloidal gold stripe was prepared and applied toto the detection of anti - PRRS antibody. Gold PRRSV, PRRSV and anti - PRRS antibody was coated on the test region and control zone respectively.Anti - PRRS antibody in 34 whole blood and sera sample was detected by the strip and PRRS - ELISA method. Result achieved from the methods mentioned above are in complete agreement. It shows that the method established in this paper bears the merit of micro analysis, specificity, conveniece and the result is easy to be tested. This method is applicable for the detection of anti - PRRS antibody in a wide area and can be applied to general survey of PRRS disease.

关 键 词：猪 繁殖呼吸综合症 抗体检测 免疫金标试纸 快速检测试纸 

分 类 号：S854.9[农业科学—临床兽医学]