血小板特异性抗体对特发性血小板减少性紫癜诊断价值的研究  

The Diagnostic Value of Platelet Glycoprotein-Specific Autoantibody Detection in Idiopathic Thrombocytopenic Purpura

作　　者：张海燕[1] 侯明[2] 张小红[1] 关向宏[1] 孙桂珍[1] 

机构地区：[1]山东省临沂市人民医院,临沂276003 [2]山东大学齐鲁医院,济南250012

出　　处：《中国实验血液学杂志》2004年第2期204-206,共3页Journal of Experimental Hematology

摘　　要：本研究的目的是比较特发性血小板减少性紫癜 (ITP)、慢性再生障碍性贫血 (CAA)、恶性血液病患者及健康志愿者特异性抗体水平 ,以评价血小板特异性抗体在ITP诊断中的价值。用改良单克隆抗体特异性俘获血小板抗原 (MAIPA)技术同时检测血小板GPIb/Ⅸ、GPIIb/Ⅲa、GPⅣ、GPⅤ的特异性抗体。结果表明 :ITP组、CAA组、恶性血液病患者及健康志愿者血小板特异性抗体总阳性率分别为 6 9.99% ,10 % ,2 0 %和 0 %。ITP组与CAA组存在显著性差异 ( χ2 =2 0 .71,P <0 .0 0 5 ) ,ITP组与恶性血液肿瘤化疗组存在显著性差异 ( χ2 =12 .2 2 ,P <0 .0 0 5 )。健康志愿组无 1例阳性。结论 :多种抗体同时检测可提高敏感性 ,血小板特异性抗体对ITP是一种特异性高、敏感性强的实验室诊断指标。To detecte levels of platelet glycoprotein specific autoantibody in idiopathic thrombocytopenic purpura (ITP), chronic aplastic anemia (CAA), hematologic malignancies and healthy volunteers, and evaluate the clinical significance of platelet glycoprotein specific autoantibody level in diagnosis for ITP, anti GPIb/Ⅸ ?anti GPIIb/Ⅲa? anti GPⅣ and anti GPⅤ auto antibodies were detected contemporaneously by a modified monoclonal antibody immobilization of platelet antigen assay (modified MAIPA). The results showed that the total positive rate of antibodies againast platelet GPIb/Ⅸ ?GPIIb/Ⅲa ?GPⅣ ?GPⅤ were 69.99%,10%,20% and 0% in ITP, CAA, hematologic malignancy group and healthy volunteers respectively. There was significant difference between ITP and CAA (χ 2=20.71, P<0.005), between ITP and hematologic malignancy group (χ 2=12.22, P<0. 005). There was no positive finding in the healthy control. It is concluded that platelet glycoprotein specific autoantibody has high value for the diagnosis of ITP,many kinds of antibodies detection at one time can enhance sensitivity, MAIPA is a specific assay for the diagnosis of idiopathic thrombocytopenic purpura.

关 键 词：特发性血小板减少性紫癜 血小板特异性抗体 血小板 

分 类 号：R558.2[医药卫生—血液循环系统疾病]