从香蕉胚性细胞悬浮系获得再生植株  

Plant Regeneration from Embryogenic Cell Suspension of Banana

作　　者：徐春香[1] 陈厚彬[1] 李建国[1] 张海岚[1] 彭国康[1] 梁铨荣[1] 

机构地区：[1]华南农业大学园艺学院热带亚热带果树研究室,广州510642

出　　处：《植物生理学通讯》2004年第2期161-163,共3页Plant Physiology Communications

基　　金：华南农业大学校长基金(5300-K02082);广东省重大专项(2002A208010102)。

摘　　要：2个主栽香蕉品种的未成熟雄花诱导产生的胚性愈伤组织接种至液体培养基中, 经3～4个月的继代培养后长成质地均匀的胚性细胞悬浮系(ECS),悬浮系中60%～80%是胚性细胞团。ECS接种至体胚再生培养基上约4～5周后开始出现再生体胚,萌发的体胚以MS培养基培养后可获得再生植株。Embryogenic calli derived from immature flowers of 2 banana cultivars (Musa spp., AAA Group)widely grown in China were used to establish embryogenic cell suspension (ECS). ECS was obtained aftercontinuous subculture and selection for 3~4 months, in which about 60%~80% was embryogenic cell aggregates.Regenerable somatic embryos emerged after the ECS was cultured on somatic embryo regeneration medium for4~5 weeks. Banana regenerated plants were obtained after the germinated somatic embryos were transferred toMS medium.

关 键 词：香蕉 胚性细胞悬浮系 再生植株 品种 液体培养基 继代培养 

分 类 号：S668.1[农业科学—果树学]