纳米氧化钛溶胶—凝胶固定葡萄糖氧化酶制备生物传感器  

Biosensor based on immobilization of glucose oxidase within an inorganic-organic hybrid TiO_2 sol-gel-derived nanocomposite

作　　者：赵滨[1] 冯学珠[2] 张婷[1] 刘宝红[1] 孔继烈[1] 吴性良[1] 

机构地区：[1]复旦大学化学系,上海200433 [2]烟台师范学院化学系,山东烟台264025

出　　处：《化学传感器》2004年第1期34-39,共6页Chemical Sensors

摘　　要：该文采用了一种新型的有机-无机复合氧化钛溶胶凝胶制备生物传感器。该方法制备的溶胶克服了通常氧化钛溶胶体系在强酸性条件下不稳定的缺点,可以通过调节pH值达到保持酶活性的目的。以葡萄糖氧化酶为例,用上述氧化钛溶胶凝胶将酶固定在玻碳电极表面,形成纳米生物复合薄膜。实验表明该传感器对葡萄糖有较好的响应特性和稳定性。同时研究了各种实验条件对氧化钛溶胶凝胶合成以及生物传感器性能的优化。A novel amperometric biosensor was fabricated based on the immobilization of glucose oxidase in an inorganic-inorganic hybrid titania sol-gel nanocomposite film. Acetylacetone worked as a complexing ligand to chalet with Ti atom in the synthesis process, and pH of the titania solution could be adjusted to the value which was optimum for retaining enzyme activity and such membrane was stably attached on the surface of glassy carbon electrode. This titania matrix could supply a good environment for enzyme loading, which resulted in sensitive monitoring of glucose in the liner range from 1.25×10^(-3) to 1.75×10^(-2) mol/L. Meanwhile, pH, temperature and the other characterization of the biosensor are also studied.

关 键 词：纳米氧化钛 溶胶-凝胶 生物传感器 葡萄糖氧化酶 制备 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]