大鼠杏仁体基底外侧核中小白蛋白反应阳性神经元受抑制性神经网络支配(英文)  

PARVALBUMIN-IMMUNOREACTIVE INTERNEURONS ARE CONTROLLED BY AN INHIBITORY NEURONAL NETWORK IN BASOLATERAL NUCLEUS OF THE RAT AMYGDALA

作　　者：李瑞锡[1] 彭裕文[1] 大谷修 西条寿夫 王劼[1] 丁忠良[1] 高璐[1] 沈馨亚[1] 

机构地区：[1]复旦大学上海医学院人体解剖与组织胚胎学系,上海200032 [2]日本国富山医科药科大学

出　　处：《神经解剖学杂志》2004年第1期12-20,共9页Chinese Journal of Neuroanatomy

基　　金：日本国文部科学省科研基金(No.12 2 10 0 0 9)资助项目

摘　　要：小白蛋白 (PV)神经元作为杏仁核簇基底外侧核 (BL)中局部神经环路成分 ,对杏仁核的情绪、学习和记忆过程等机能发挥重要作用。为探讨 BL中 PV中间神经元的突触形成状态 ,本研究用抗 PV抗体标示 PV神经元 ,以抗多巴胺 (DA)抗体标示多巴胺能轴突及末梢作为传入纤维的标志 ,对大鼠杏仁核做了免疫电镜双标记研究。结果表明 ,突触主要见于 PV免疫阳性神经元的树突结构上 ,包括从树突干到中间及小型树突的各级分支。其中 68%的突触由未标记的轴突终末形成 ,3 2 %分别由 DA(2 1% )和 PV(11% )免疫阳性轴突末梢形成。 PV免疫阳性神经元与未标记末梢所形成的突触大多数是对称性的 ,仅少数为非对称性。这些非对称性突触见于 PV神经元的树突小棘和连续性突触 ,即一个未标记轴突末梢与另一个未标记轴突末梢形成对称性突触 ,后者又与 PV免疫阳性神经元树突形成非对称性突触。 DA和 PV免疫阳性神经元轴突终末与 PV免疫阳性神经元树突之间的突触全部是对称性的。以上结果表明 ,大鼠杏仁核 BL 的As the elements of local neuronal circuits, parvalbumin (PV) containing interneurons in the basolateral nucleus (BL) of the amygdala play an important role in the amygdaloid functions of emotion, learning and memory. In order to investigate how the PV containing interneurons in the BL are controlled, the synapses established on PV containing interneurons in the BL of the rat amygdala were examined under immunoelectron microscopy using the double labeling methods with anti PV and anti dopamine (DA) antibodies for a reference of dopaminergic axon terminals. The results show that the PV immunoreactive (IR) neurons formed the synapses mainly on the dendritic structures from shafts of the dendrites to median and small dendritic branches. 68% of the synapses on the PV IR profiles were formed by unlabeled axon terminals, and 32% of them were formed by DA (21%) and PV (11%)IR axon terminals. Majority of the synapses on the PV IR neurons formed by unlabeled axon terminals were symmetric type, and only a small a mount of them were asymmetric that were observed between the PV IR spines and unlabeled axon terminals and in the serial synapses in which an unlabeled axon terminal symmetrically contacted to another unlabeled axon terminal that, in turn, synapsed asymmetrically to the PV IR dendritic profiles. The synapses formed between the PV IR profiles and DA or PV IR axon terminals were exclusively symmetric. The present results suggest that the PV containing interneurons in the BL of the rat amygdala were controlled by an inhibitory network formed by the symmetric synapses around them, among which the DA system was included.

关 键 词：小白蛋白中间神经元 大鼠 免疫电镜 杏仁核 多巴胺能神经末梢 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]