卤虫(Artemia salina)孵化腺细胞分化时相的免疫细胞化学研究(简报)  

IMMUNOCYTOCHEMICAL STUDIES ON THE PHASE OF DIFFERENTIATION OF HATCHING GLAND CELLS IN BRINE SHRIMP, ARTEMIA SALINA

作　　者：李凌[1] 樊廷俊[1] 汪小锋[1] 丛日山[1] 于秋涛[1] 钟其旺[1] 

机构地区：[1]中国海洋大学生命科学与技术学部海洋生物系,青岛266003

出　　处：《实验生物学报》2004年第2期157-164,共8页Acta Biologiae Experimentalis Sinica

基　　金：国家教育部留学回国人员基金(No.980418)

摘　　要：动物孵化酶(hatching enzyme,HE)是早期胚胎在特定发育阶段由孵化腺细胞产生和分泌的,在动物早期胚胎孵化中具有关键性作用[1-4].孵化腺细胞(hatching gland cell,HGC)一般为单细胞腺体,是从胚胎发育到特定阶段(孵化前)出现、至胚胎孵出后的特定时期消失的一时性细胞(transient type 0fcells)[5-7].Hatching enzyme (HE), synthesized in hatching gland cells (HGCs), plays vital roles in animal hatching. Immunocytochemical techniques employing anti-GST-UVS.2 antiserum, prepared from Xenopus HE and with specificity to brine shrimp HE, were first used to investigate the differentiation and variability of hatching gland cells (HGCs) in the hatching process of embryos of brine shrimp, Anemia salina, in this study. HGCs with immunoreactivity to anti-GST-UVS.2 antiserum were identified, for the first time, in brine shrimp embryos during hatching process. Immunocytochemical staining results showed that, (1 )HE-positive immunoreactivity is really specific to Artemia HE, and its appearance and disappearance are closely correlated with the hatching process of Artemia salina.(2)Artemia HGCs, first appeared in embryos 5 hours before hatching and disappeared 4 hours after hatching, were also a transient type of cells, with an existence period of 9 hours. (3)The head portion of Artemia embryo is probably the initial position of HE secretion, and likely to be the main position of HE secretion as well. The detailed process and mechanism need to be studied. (4)The appearance of HGCs is in a synchronous mode from places all over the embryos, and their disappearance is also in a synchronous mode.(5)The number of HGCs increased gradually along with embryo development process and reached a maximum number at hatching. Contrarily, the number of HGCs decreased gradually after hatching, and HGCs disappeared 5 hours after hatching. However, the intensity of HE-positive reaction was almost at the same level at the period of HGCs'presence. (6)Artemia HGCs were distributed throughout the body of embryos at all time during their presence. Therefore, it can concluded that Artemia HGCs, as a transient type of cells, first appeared in embryos 4 hours before hatching and disappeared in embryos 5 hours after hatching, and with distinguished patterns of appearance, disappearance and distribution in embryos. What is the final destiny of Artemia HG

关 键 词：卤虫 孵化酶 孵化腺细胞 免疫细胞化学 分化时相 盐水丰年虫 

分 类 号：Q45[生物学—生理学]