人胎胰巢蛋白阳性细胞的异种体内移植研究  

ALLOGENIC TRANSPLANTATION STUDIES ON HUMAN FETAL PANCREATIC NESTIN POSITIVE CELLS

作　　者：黄海霞[1] 黄心智[1] 陈新宇[1] 陈玉英[1] 汤雪明[1] 

机构地区：[1]上海第二医科大学细胞生物学教研室,上海200025

出　　处：《实验生物学报》2003年第3期185-190,共6页Acta Biologiae Experimentalis Sinica

摘　　要：人胎胰巢蛋白阳性(nestin^+)细胞在体外培养中可自发形成类胰岛细胞团(islet-1ike cell cluster,ICC),有多向分化潜能,并可产生分泌胰岛素的类β细胞。为了验证其体内生物学特性和生理功能,我们进行了诱导后ICC的NOD-Scid糖尿病模型小鼠和正常小鼠肾膜下移植,通过免疫细胞化学检测,超微结构观察以及血糖水平监测等手段考察移植后细胞的形态与功能变化情况,结果表明:(1)移植处有明显的血管增生。(2)ICC可使糖尿病模型小鼠血糖明显降低。(3)ICC在正常小鼠体内分化为多种结构,同时继续增殖侵入肾实质。Human fetal pancreatic nestin positive cells cultured in vitro could self assemble to form islet-like cell clusters (ICC). They are multipotential and are capable of generating insulin-producing cells. To investigate their biological character and physiological function in vivo, ICCs were implanted into NOD-Scid mice subcapsularly. The results showed that: (1) Neovascularizations were observed in implant sites. (2) Blood glucose levels of diabetic mice were reduced markedly after implantation of ICCs. (3) ICCs in non-diabetic mice proliferated abnormally and infiltrated into renal parenchyma with many cell structures formed.

关 键 词：人胎胰巢蛋白阳性细胞 胰腺 NESTIN ICC 移植 血管 增生 糖尿病 

分 类 号：R587[医药卫生—内分泌]