利用氧化剂甲基紫精筛选拟南芥寿限延长突变体  

SCREENING FOR LIFESPAN-EXTENSION MUTANTS WITH PARAQUAT IN ARABIDOPSIS

作　　者：曹树青[1] 徐巧婷[1] 周红军[1] 曹雅君[1] 朱勇[1] 俞芳[1] 蒯本科[1,2] 

机构地区：[1]复旦大学生命科学学院生物化学系 [2]上海 200433

出　　处：《实验生物学报》2003年第3期233-237,共5页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金39870452;上海市科技发展基金003913004;023112039;复旦大学985学科建设交叉学科资助项目X-8

摘　　要：在模式动物中的研究表明,寿限延长与氧化胁迫耐受能力密切有关;在模式植物拟南芥中也发现晚花突变体具有更强的抗氧化胁迫能力,但迄今为止未见有关拟南芥寿限延长突变体的研究报道。本文建立了用氧化剂甲基紫精筛选寿限延长突变体的方法,并对用该方法从经快中子诱变的拟南芥Columbia生态型M_2代群体中筛选获得的突变体SFNA-9-4进行分析,发现该突变体的抗氧化胁迫能力和寿限均显著增加。由此说明,用该方法筛选拟南芥寿限延长突变体是可行的。Genetic analyses of lifespan in model animals have revealed that extended lifespans are closely associated to increased resistance to oxidative stress. In the model plant Arabidopsis , late-flowering mutants are also found to be more tolerant to oxidative stress. However, Arabidopsis mutants with extended lifespans are poorly studied so far. In this study, a screening system for mutants with extended lifespans in Arabidopsis was established using paraquat, a potent herbicide that exacerbates O2- radical production. The relationship between lifespan and resistance to oxidative stress was examined with one of the mutants, SFNA-9-4. Compared to that of wild type, the lifespan of SFNA-9-4 is significantly extended, and its resistance to oxidative stress is also significantly elevated. These results suggest that, as in Drosophila , paraquat can also be used to screen for mutants with extended lifespans in Arabidopsis.

关 键 词：氧化剂 甲基紫精 筛选 突变体 拟南芥 衰老 植物 寿限 

分 类 号：Q945[生物学—植物学]