一株耐热红色毛癣菌临床分离株的基因型鉴定  

Granulomatous Skin Lesion Due to Hot-resistant Trichophyton rubrum and Its Variation in Molecular Biology

作　　者：章强强[1] 李民[1] 朱利平[1] 李莉[1] 王家俊[1] 

机构地区：[1]上海复旦大学附属华山医院,200040

出　　处：《中华皮肤科杂志》2003年第8期443-445,共3页Chinese Journal of Dermatology

摘　　要：目的探讨1株引起特殊皮肤肉芽肿损害的红色毛癣菌菌株的真菌学特点及其基因序列的变化。方法常规真菌学鉴定法和使用PCR方法测定菌株的核糖体保守区及非转录区(NTS)内trs-1区基因序列。结果耐热菌株经真菌学鉴定及核糖体保守区基因序列测定均证实为红色毛癣菌,但其核糖体基因非转录区(NTS)内trs-1(串联重复亚单位)区基因序列测定显示有明显的不同。结论核糖体基因NTS区内trs-1区基因序列的改变可能的解释为此株耐热红色毛癣菌为红色毛癣菌的一个同型变种,这一变种具有较强的侵袭力,能导致特殊的皮肤损害;亦或为菌株为适应外界环境条件改变发生的基因水平的变异。Objective To investigate the mycologic features and variation in gene sequence of a hot-resistant Trichophyton rubrum which caused granulomatous skin lesion.Methods The isolate was identified by routine and polymerase chain reaction(PCR)technique.Ribosomal conserved sequence and trs-1(tandem repetitive subelement)sequence in the ribosomal non-transcribed spacer(NTS)were determined.Results The hot-resistant isolate was identified as T.rubrum by routine and PCR technique.The trs-1sequence variation in rDNA NTS was found in the strain.Conclusion The trs-1sequence variation suggests that this strain be one of isotypic variants of T.rubrum,which has much higher pathogenicity to cause granulomatous skin lesion,or sequence variation of the strain may be the result of adapting itself to environmental change(37℃),which needs further studies.

关 键 词：耐热红色毛癣菌 基因型 Majocchi肉芽肿 序列分析 发癣菌属 病历资料 

分 类 号：R756[医药卫生—皮肤病学与性病学]