阴道念珠菌随机扩增多态性DNA分型研究  

Genotyping of Candida Strains from Patients with Vulvovaginal Candidiasis by Use of Random Amplified Polymorphic DNA

作　　者：张晓梅[1] 骆志成[1] 武三卯[1] 薛晓云[1] 周晓黎[1] 石磊[2] 肖瑾[3] 

机构地区：[1]兰州医学院第二附属医院皮肤科 [2]兰州医学院第二附属医院检验科 [3]兰州医学院第二附属医院妇产科

出　　处：《中华皮肤科杂志》2003年第8期449-451,共3页Chinese Journal of Dermatology

基　　金：甘肃省自然科学基金项目(ZS011-A25-073-Y)

摘　　要：目的对分离自外阴阴道念珠菌病患者阴道的念珠菌进行基因分型,并对不同菌株间的亲缘关系作相似性分析.方法用形态学及生化方法对分离到的致病性念珠菌进行鉴定,然后采用随机扩增多态性DNA技术(RAPD)对45株临床分离株基因组DNA进行扩增,并将DNA扩增带型进行聚类分析.结果30株白念株菌可以分成7群共19个亚群,15株非白念珠菌(包括光滑念珠菌6株,克鲁斯念珠菌、近平滑念珠菌和热带念珠菌各3株)至少可鉴定到种水平.白念珠菌种内不同菌株间的相似性系数在90%以上,不同念珠菌种间的相似性系数介于80%～90%.结论外阴阴道念珠菌病的主要致病菌是白念珠菌,用RAPD技术可将之分成不同的基因组型别.Objective To genotype Candida strains by random amplified polymorphic DNA(RAPD)technique,and to analyse the analogy of different Candida strains based on similarity coefficient.Methods Candida strains were isolated from vaginal secretion taken from patients with vulvovaginal can-didiasis by Sabouraud' s dextrose agar,C.albicans was differentiated from non-C.albicans strains by germ tube test,chlamydospore test and CHROMagar-Candida,and further identified by API20c Aux test Kits.The genomic DNA of45Candida isolates,including Candida albicans,Candida glabrata,Candida krusei,Candida parapsilosis and Candida tropicalis were amplified by RAPD,and DNA polymorphisms of inter-species and intraspecies were evaluated by using the DNA pattern-clustering of5primers.Results Thirty strains of C.albicans could be classified into7groups,and further divided into19subgroups,while15strains of non-C.albicans could be identified to species level.C.albicans isolates were related to each other with the similarity coefficients of more than90%,while different Candida species were connected to each other with the similarity coefficients of80%~90%.Conclusion C.albicans,which is the predominant pathogen of vulvovaginal candidiasis,could be classified into different genotypes by use of RAPD method.

关 键 词：阴道念珠菌 DNA分型 基因多态性 外阴阴道 念珠菌病 VVC 流行病学 

分 类 号：R346[医药卫生—基础医学]