精氨酸加压素对血管内皮细胞分泌VEGF的影响  

EFFECT AND MECHANISM OF ARGININE VASOPRESSION (AVP) ON SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) BY ENDOTHELIAL CELLS

作　　者：李中言[1] 陈丹[1] 叶季鲜[1] 薛静[1] 

机构地区：[1]第四军医大学吉林军医学院附属医院,吉林132013

出　　处：《解放军医学杂志》2003年第9期819-820,共2页Medical Journal of Chinese People's Liberation Army

摘　　要：为观察精氨酸加压素(AVP)对血管内皮细胞 (VEC)分泌血管内皮细胞生长因子 (VEGF)的影响 ,以培养的人大网膜VEC为模型 ,检测不同浓度AVP作用下VEGF浓度及蛋白激酶C(PKC)活性。结果显示 ,1× 10 -91× 10 -7mol/LAVP作用 6h后 ,VEGF浓度及PKC活性较对照组明显升高 (P<0 0 1)。提示AVP呈浓度依赖性地刺激VEC分泌VEGF ,PKC可能介导了AVP刺激VEC分泌VEGF的作用。To investigate the effect of arginine vasopression (AVP) on the secretion of vascular endothelial growth factor (VEGF) by vascular endothelial cell (VEC). VEC of human greater omentum artery were isolated and cultured. The concentrations of VEGF and activity of protein kinase C (PKC) in the culture medium were measured. Results indicated that after the cells were incubated with 1×10 -9～1×10 -7mol/L AVP for 6 hours, the concentration of VEGF and activity of PKC were markedly increased compared to control (P<0.01). It suggested that AVP could induce VEGF to secrete VEC in a dose-dependent way, meanwhile PKC could regulate the effect of AVP to secrete VEGF.

关 键 词：精氨酸加压素 内皮生长因子 内皮 血管 

分 类 号：R541.302[医药卫生—心血管疾病]