不同激素对花生离体分化的影响  

Effects of different plant hormones In-vitro differentiation of peanut

作　　者：梁丽琨[1] 林荣双[1] 由翠荣[1] 王庆华[1] 肖显华[1] 

机构地区：[1]烟台大学生化系,烟台264005

出　　处：《植物研究》2004年第2期187-191,T011,共6页Bulletin of Botanical Research

基　　金：山东省自然科学基金资助项目 (Y97D160 75 )~~

摘　　要：对TDZ和 2 ,4 D等激素在花生成熟胚外植体分化中的影响进行了研究。结果表明 ,花生成熟胚 3～ 5d龄实生苗的幼叶和胚轴在低浓度TDZ的诱导下 ,可分化产生高频不定芽和少量体细胞胚 ,转到无激素MS培养基或MS +BA 0 .5mg/L+NAA 0 .4mg/L的培养基后形成丛生苗。丛生苗分离后转入含 1 /2MS(大量元素 ) +IBA 0 .4mg/L的培养基中诱导生根 ,可形成完整的再生植株。幼叶分化率高于胚轴 ,但胚轴分化成苗速度快。无菌水浸泡 1 6～ 2 4h的胚轴在 5～30mg/L2 ,4 D的诱导下 ,分化产生低频不定芽 ;而胚叶则产生高频体细胞胚 。The effects of TDZ and 2,4-D on the differentiation of mature peanut (Arachis hypogaea L.) embryonic explants were investigated. The results showed that leaflets and embryonic axes of 3～5-day-old seedlings produced multiple buds and somatic embryos, which developed into shoots following transferred to MS medium without hormone or MS medium supplemented with BA0.5 mg/L and NAA 0.4 mg/L. These shoots regenerated normal plants after they were transferred to medium containing half strength major elements and 0.4 mg/L IBA. The differentiation rate of leaflets is higher than that of embryonic axes while the latter produced regenerated plants in a shorter period of time. Embryonic axes soaked in sterile water for 16～24 h could form adventitious buds with low frequency on medium containing 5～30 mg/L 2,4-D and young leaflets of embryos differentiated somatic embryos with abnormality on the induction of 5～30 mg/L 2,4-D.

关 键 词：激素 花生 成熟胚 体细胞胚 TDZ 2 4-D 外植体 分化 

分 类 号：S565.201[农业科学—作物学]