天然红松遗传多样性在时间尺度上变化的RAPD分析  

RAPD analysis on variation of genetic diversity of natural Pinus koraiensis in temporal dimension

作　　者：张恒庆[1] 刘德利[1] 金荣一[1] 吕建洲[1] 王青[1] 

机构地区：[1]辽宁师范大学生命科学学院,大连116029

出　　处：《植物研究》2004年第2期204-210,共7页Bulletin of Botanical Research

基　　金：国家自然科学基金 ( 3 0 170 176)资助项目

摘　　要：应用RAPD技术对凉水国家自然保护区的天然红松 (Pinuskoraiensis)种群在时间尺度上的遗传多样性变化和遗传分化进行了分析。选择树龄为 1～ 1 0 0a的红松个体 ,每 1 0a为 1个龄级 ,共采集样本 2 4 5个。 1 0个随机引物共检测到 61个位点 ,其中多态位点 5 0个 ,多态位点比率为 81 .97%。Shannon指数龄级间遗传多样性占总多样性的 1 6.43% ,Nei指数龄级间遗传分化为 1 3.64% ,两种指数都表明 ,红松在时间尺度上的遗传分化比空间尺度的低 ,红松的遗传变异主要存在于龄级内。本研究结果证实 ,凉水保护区内的红松遗传多样性在近 1 0 0a的时间内出现过大小两次波动 ,目前该地区的红松遗传多样性正处于上升时期 ;历史上小规模的采伐虽然暂时降低了红松的遗传多样性 ,但由于保护区的及时建立 。With RAPD method,the paper studied genetic diversity and differentiation in temporal dimension of natural population of Pinus koraiensis in Liangshui National Nature Reserve. Total 245 P.Koraiensis samples aged from 1 to 100 years were grouped 10 age classes every 10 years. 61 repeatable loci in which 50 were polymophic were obtained from 10 random primers and percentage of polymophic loci was 81.97%. In age class level, genetic differentiation of Shannon diversity index and Nei index was 16.43% and 13.64%. Two indexes showed genetic differentiation in temporal dimension is lower than in space and genetic variation existed within age class. Result showed that genetic diversity of P.koraiensis in Liangshui national nature reserve fluctuated twice in 100 years and is ascending at present. Though genetic diversity of P.koraiensis decreased temporarily after some small fellings, it has restored effectively because of establishment of the nature reserve.

关 键 词：红松 RAPD 遗传多样性 时间尺度 自然保护区 森林树种 

分 类 号：S791.247[农业科学—林木遗传育种]