德国小蠊抗药性检测方法比较研究  

Comparisons of Two Methods for Insecticide Resistance Detection to the German Cockroach, Blattella germanica

作　　者：曾晓芃[1] 薛素琴[1] 付学锋[1] 

机构地区：[1]北京市疾病预防控制中心,北京100013

出　　处：《中国媒介生物学及控制杂志》2004年第1期17-19,共3页Chinese Journal of Vector Biology and Control

基　　金：北京市自然科学基金资助项目 (NO .7992 0 2 4 )

摘　　要：目的　对德国小蠊两种常用抗性检测方法进行比较 ,以了解不同方法在抗性系数 (RR)上的差异 ,为根据需要选择适宜的抗性检测方法提供帮助。方法　分别参照WHO和Atkinson等 (1991)及Zhai等 (1992 )的方法。结果　用药膜法测定KT50 ,德国小蠊对二氯苯醚菊酯、溴氰菊酯和氯氰菊酯的抗性倍数分别是 6.93、13 .85和 5 .60倍 ;而用点滴法测定LD50 ,德国小蠊对上述 3种药剂的抗性倍数分别为 2 2 .17、64 .3 2和 2 7.0 3倍。可见 ,用点滴法比用药膜法测定德国小蠊对二氯苯醚菊酯、溴氰菊酯和氯氰菊酯的抗性系数分别要高出 3 .2 0倍、4.64倍和 4.83倍。结论　点滴法较药膜法更为灵敏 ,能较为准确地反映出德国小蠊的实际抗性水平 ,而药膜法相对简单易行 ,适于现场抗性检测。Objective To compare difference of two methods for insecticide resistance detection in the resistance ratio of the German Cockroach, Blattella germanica. Methods Insecticide resistance was detected by methods of WHO and Atkinson & Zhai respectively. Results The resistance ratio (RR) measured by surface contact was 6.93 folds for permethrin, 13.85 for deltamethrin and 5.60 for cypermethrin based on KT 50. As measured by topical application, the resistance ratio based on LD 50 was 22.17 folds for permethrin, 64.32 for deltamethrin and 27.03 for cypermethrin. It means that the resistance ratios of this insect to permethrin, deltamethrin and cypermethrin by topical application were 3.20,4.64 and 4.83 times higher than that by surface contact method. Conclusion The results suggested that surface contact method do not accurately assess insecticides susceptibility in field populations of German cockroaches, topical application was more sensitive than surface contact tests for measuring insecticide resistance.

关 键 词：德国小蠊 抗药性 检测方法 药膜法 点滴法 药剂 

分 类 号：R384.1[医药卫生—医学寄生虫学]