一种简单通用的鸟类性别分子鉴定技术(简报)  

A SIMPLE AND UNIVERSAL METHOD FOR MOLECULAR SEXING OF BIRDS

作　　者：胡锐颖[1] 耿昕[1] 马珺[2] 陈云霜[2] 李仲逵 丁小燕[1] 

机构地区：[1]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031 [2]上海动物园,上海200335

出　　处：《实验生物学报》2003年第5期401-404,共4页Acta Biologiae Experimentalis Sinica

基　　金：中国科学院创新经费;上海市绿化管理局(010419)资助

摘　　要：我国幅员辽阔,生物类型多种多样,是世界上拥有鸟类种类最多的国家之一.截至1999年底,已知有鸟类1253种948亚种,隶属于21目83科,其中有多种为我国特有或珍稀濒危的鸟类[1,2].It is difficult to sex monomorphic or juvenile birds only according to their appearance or by simple surgical techniques. However, the sex of individual bird has to be identified during breeding. Recently, a sex-linked gene, CHD1, has been discovered in non-ratite birds. Using highly conserved primers flanking the intron of CHD1 and PCR amplification, females are characterized by di-aplaying two fragments( CHDIW and CHDIZ), while males only show one fragment( CHDIZ) clearly different in size from the female -specific CHDIW fragment. PCR-based molecular sexing method has been widely used due to its many merits over other techniques. In this work, we successfully sexed 86 bird from 9 species using this technique. All of these birds are listed here as the national first-grade wildlife of China for protection. Our results benefit greatly on breeding rare monomorphic birds and preventing them from distinction.

关 键 词：鸟类 性别 分子鉴定技术 聚合酶链式反应 单态性鸟 

分 类 号：Q41[生物学—生理学]