Ketamine suppresses intestinal NF-kappa B activation and proinflammatory cytokine in endotoxic rats  

作　　者：JieSun Xiao-DongWang HongLiu Jian-GuoXu 

机构地区：[1]DepartmentofAnesthesiology,JinlingHospital,CollegeofMedicine,NanjingUniversity,Nanjing210002,JiangsuProvince,China [2]DepartmentofSurgery,JinlingHospital,CollegeofMedicine,NanjingUniversity,Nanjing210002,JiangsuProvince,China [3]DepartmentofChestSurgery,JinlingHospital,CollegeofMedicine,NanjingUniversity,Nanjing2i0002,JiangsuProvince,China

出　　处：《World Journal of Gastroenterology》2004年第7期1028-1031,共4页世界胃肠病学杂志（英文版）

摘　　要：AIM: To investigate the protective effect of ketamine on the endotoxin-induced proinflammatory cytokines and NFkappa B activation in the intestine. ETHODS: Adult male Wistar rats were randomly divided into 6 groups: (a) normal saline control, (b) challenged with endotoxin (5 mg/kg) and treated by saline, (c) challenged with endotoxin (5 mg/kg) and treated by ketamine (0.5 mg/kg), (d) challenged with endotoxin (5 mg/kg) and treated by ketamine (5 mg/kg ), (e) challenged with endotoxin (5 mg/kg) and treated by ketamine (50 mg/kg), and (f) saline injected and treated by ketamine (50 mg/kg). After 1, 4 or 6 h, TNF-α and IL-6 mRNA were investigated in the tissues of the intestine (jejunum) by RT-PCR. TNF-α and IL-6 were measured by ELISA. We used electrophoretic mobility shift assay (EMSA) to investigate NF-kappa B activity in the intestine. RESULTS: NF-kappa B activity, the expression of TNF-α and IL-6 were enhanced in the intestine by endotoxin. Ketamine at a dose of 0.5 mg/kg could suppress endotoxininduced TNF-α mRNA and protein elevation and inhibit NFkappa B activation in the intestine. However the least dosage of ketamine to inhibit IL-6 was 5 mg/kg in our experiment. CONCLUSION: Ketamine can suppress endotoxin-induced production of proinflammatory cytokines such as TNF-α and IL-6 production in the intestine. This suppressive effect may act through inhibiting NF-kappa B.AIM:To investigate the protective effect of ketamine on the endotoxin-induced proinflammatory cytokines and NF- kappa B activation in the intestine. METHODS:Adult male Wistar rats were randomly divided into 6 groups:(a) normal saline control,(b) challenged with endotoxin (5 mg/kg) and treated by saline,(c) challenged with endotoxin (5 mg/kg) and treated by ketamine (0.5 mg/kg), (d) challenged with endotoxin (5 mg/kg) and treated by ketamine (5 mg/kg),(e) challenged with endotoxin (5 mg/kg) and treated by ketamine (50 mg/kg),and (f) saline injected and treated by ketamine (50 mg/kg).After 1,4 or 6 h,TNF-α and IL-6 mRNA were investigated in the tissues of the intestine (jejunum) by RT-PCR.TNF-α and IL-6 were measured by ELISA.We used electrophoretic mobility shift assay (EMSA) to investigate NF-kappa B activity in the intestine. RESULTS:NF-kappa B activity,the expression of TNF-α and IL-6 were enhanced in the intestine by endotoxin. Ketamine at a dose of 0.5 mg/kg could suppress endotoxin- induced TNF-α mRNA and protein elevation and inhibit NF- kappa B activation in the intestine.However the least dosage of ketamine to inhibit IL-6 was 5 mg/kg in our experiment. CONCLUSION:Ketamine can suppress endotoxin-induced production of proinflammatory cytokines such as TNF-α and IL-6 production in the intestine.This suppressive effect may act through inhibiting NF-kappa B.

关 键 词：克他命 NF-κB 炎症 内毒素 动物实验 药理作用 肠道功能 细胞因子 脂多糖 

分 类 号：R512.5[医药卫生—内科学] R971.2[医药卫生—临床医学]