转染HCV核心基因后对人胆管癌细胞中hTERT mRNA表达的影响  

The effect of HCV C gene transfection on expression of human telomerase reverse transcriptase(hTERT)mRNA in human biliary carcinoma cell lines.

作　　者：李志花[1] 陈汝福[1] 谢德荣[1] 林显敢[1] 姚和瑞[1] 陈岱佳[1] 梁新文 刘天浩[1] 江志敏[1] 

机构地区：[1]中山大学附属第二医院,广东广州510120

出　　处：《现代肿瘤医学》2004年第2期86-89,共4页Journal of Modern Oncology

基　　金：中国博士后科学基金资助项目 ( 2 0 0 2 0 3 12 91)

摘　　要：目的　探讨丙型肝炎病毒核心基因 (hepatitisCviruscoregene ,HCVC基因 )调控端粒酶逆转录酶(humantelomerasereversetranscriptase,hTERT)基因表达在肝门部胆管癌发生中的作用。方法　将构建成功的HCVC基重组真核表达载体pcDNAHCV -C和空载体与绿色荧光蛋白基因共转染人胆管正常上皮细胞 (hu mannormalbiliaryepithelialcells ,BEC)和胆管癌细胞QBC939,RT -PCR和免疫组织化学方法分析转染后细胞内hTERTmRNA和蛋白的表达。结果　pcDNAHCV -C在BEC和QBC939细胞中的转染率为 16 %和 32 % ,转染HCVC基因的BEC组表达hTERTmRNA ,而转染空载体的BEC不表达hTERTmRNA ;表达HCVC蛋白QBC939中hTERTmRNA和蛋白的表达明显高于空载体组。结论　转染的HCVC基因可以上调hTERT基因的表达 。Objective To study the effect of human telomerase reverse transcriptase (hTERT)mRNA gene expression controlled by hepatitis C virus core gene(HCV C gene)on occurrenc of human biliary carcinoma.Methods The eukaryotic expression vector recombination with HCV C gene(pcDNA3-HCVC)and the vector-alone were co-transfected with enhanced green fluorescent protein(EGFP)into biliary carcinoma cell(QBC939)and human normal biliary epithelial cells(HBEC).The reverse transcription PCR(PT-PCR)and immunohistochemical methed were used to analyze the expression of hTERT mRNA and protein.Results The transfection efficiency of pcDNAHCV-C,were about 16%and 32% in QBC939 and HBEC.The HBEC transfected by HCV C gene express hTERT mRNA and there was no expression of hTERT mRNA in HBECs when transfected blank vector,but a dramatic phenomenon was observed that the expression of hTERT mRNA and protein assayed in QBC939 was more strikingly increased when transfected with HCV C expression vector than that transfected with blank vector.Conclusion HCV C gene transfection can up-regulate the expression of hTERT mRNA in biliary cancinoma cells,it maybe a way the HCV gene evoke biliary carcinoma.

关 键 词：HCV核心基因 胆管癌细胞中 端粒酶逆转录酶 基因转染 真核表达载体 RT-PCR 免疫组织化学 

分 类 号：R373.21[医药卫生—病原生物学] R735.8[医药卫生—基础医学]