牛珀至宝微丸对内毒素休克大鼠心脏诱导型一氧化氮合酶表达的影响  

Influence of Niupozhibao pellet(牛珀至宝微丸) on inducible nitric oxide synthase expression of heart in endotoxic shock rat

作　　者：黄彬[1] 杜少辉[1] 陈东风[2] 黎晖[2] 李伊为[2] 魏志军[1] 

机构地区：[1]深圳市中医院,广东深圳518033 [2]广州中医药大学解剖教研室,广东广州510405

出　　处：《中国中西医结合急救杂志》2004年第2期95-98,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目 ( 3 9970 914 ) ;广东省中医药管理局资助项目 ( 9844 7);深圳市科技局资助项目 ( 2 0 0 0 0 40 87)

摘　　要：目的 :探讨牛珀至宝微丸对内毒素休克大鼠心脏诱导型一氧化氮合酶 (i NOS)表达的影响。方法 :静脉注射内毒素 (L PS) 1.5 mg/ kg、腹腔注射 D 氨基半乳糖 (D Gal N) 10 0 mg/ kg造成内毒素休克模型 ,采用免疫组织化学方法检测心脏 i NOS的表达。结果 :内毒素组 i NOS阳性细胞分布于心脏外膜与心肌。牛珀至宝微丸能明显降低内毒素所致 i NOS的表达。结论 :牛珀至宝微丸治疗内毒素休克的心脏损伤与其下调 i NOS的表达相关。Objective: To investigate the effect of Niupozhibao pellet(牛珀至宝微丸) on inducible nitric oxide synthase(iNOS) expression of heart in endotoxic shock rat. Methods: The model of endotoxic shock was induced by 1.5 mg/kg of lipopolysaccharide(LPS) intravenously injection and 100 mg/kg D-galactosamine (D-GalN) intraperitoneally injection. The expression of iNOS was measured by immunohistochemistry. Results: iNOS immuno-positive cells in LPS group distributed in myocardium and epicardium. The number of positive cells in LPS group were much more than those of Niupozhibao pellet treatment group(P<0.01). Conclusion: Niupozhibao pellet can improve the injury of heart induced by endotoxic shock through decreasing iNOS expression.

关 键 词：牛珀至宝微丸 内毒素休克 大鼠 心脏 诱导型一氧化氮合酶 表达 

分 类 号：R285[医药卫生—中药学]