用PCR法从大肠杆菌K12菌株中克隆tyrB基因  

The Cloning of TyrB Gene from Escherichia Coli K12 by PCR Technology

作　　者：史燕东[1] 吴梧桐[1] 王旻 张玉彬 

机构地区：[1]中国药科大学生物技术研究中心

出　　处：《中国药科大学学报》1992年第6期361-363,共3页Journal of China Pharmaceutical University

摘　　要：用PCR法从大肠杆菌K12菌株中调出一个长约1.2 kb的DNA片段,该片段插入pKK233-2表达载体后转化tyrB^-菌JP4282,得到3株tyrB^+菌,从3株tyrB^+菌中提取的质粒可用限制性内切酶切出一个1.2 kb长的插入片段,证明该片段具有tyrB基因活性。By using the PCR technology, we obtained a 1.2kb DNA fragment from E. coli K 12. This fragment was inserted into Nco Ⅰ site of pKK233-2. The recombinant plasmid was used to transform tyrB mutant JP4282. Three tyrB^+ strains from 900 transformants were obtained. The three tyrB^+ strains were named: cpuJP-tyrB^+ 9204-1,2,3 respectively. The plasmids, extracted from cpuJP-tyrB^+ 9204, can cleave out a 1.2kb fragment with restriction enzyme Nco Ⅰ.

关 键 词：PCR法 克隆 大肠杆菌 

分 类 号：TQ460.1[化学工程—制药化工]