磷酸川芎嗪与大鼠肝微粒体相互作用的研究  

STUDY ON THE INTERACTIONS BETWEEN TETRAMETHYLPYRAZINE PHOSPHATE AND LIVER MICROSOMES IN RATS

作　　者：李峰[1] 楼雅卿[2] 

机构地区：[1]天津医学院附属医院神经病学研究所,300052 [2]北京医科大学药理教研室,北京100083

出　　处：《中国药理学通报》1992年第1期32-35,共4页Chinese Pharmacological Bulletin

摘　　要：Sprague-Dawley6大鼠ig50、100和300mg/kg磷酸川芎嗪,对肝微粒体细胞色素P-450酶系无显著性影响。苯巴比妥诱导P-450b后再ig磷酸川芎嗪,其体内血药浓度明显低于正常对照组,说明川芎嗪在体内代谢可能和肝微粒体细胞色素P-450酶系统中的P-450b同功酶有关。The two methods of preparing rat liver microsomes were compared. The microsome yield by ultracentrifugation was far grater than that by Ca2+ aggregation(P<0.01)while the content of liver cy-tochrome P-450 was not significantly different from each other ( P> 0.05) . The results showed that the ig treatment of TMPP at the doses of 50, 100 and 300 mg/kg, respectively, once daily for 10d, had no significant effects on the liver weight, microsome yield, cytoch-rome P-450, Cytochrome b5 and NADPH Cyt C reductase. It was indicated that TMPP metabolized by liver had no ability to induce or inhibit the cytochrome P-450 enzymes in rats. Pretreatment with phenobarbital (75mg/kg·d-1, for 3d ) , a mainly P-450b inducer, significantly decreased the plasma concentrations of TMPP in rats. Therefore, it is suggested that the metabolism of TMPP in rats is probably related to the cytochrome P-450b of liver.

关 键 词：磷酸川芎嗪 苯巴比妥 肝微粒体 

分 类 号：R965[医药卫生—药理学]