(一)蝙蝠葛苏林碱对兔淋巴细胞内钙含量的影响  

The effect of ( - )Daurisoline on cytoplasmic free calcium concentration of rabbit lymphocytes

作　　者：程锦轩[1] 张一彬[1] 段全虹 朝凤云 胡韶毅[1] 吴亭[2] 王振纲[1] 

机构地区：[1]中国医学科学院基础医学研究所药理室,北京100005 [2]国家计划生育委员会计划生育研究所,北京100014

出　　处：《中国药理学通报》1992年第3期198-201,共4页Chinese Pharmacological Bulletin

摘　　要：采用钙荧光指示剂Fura—2/Am定量测试法检测了静息淋巴细胞胞浆内游离钙浓度,并以Ca^(2+)载体A23187作为阳性对照.观察了(一)蝙蝠葛苏林碱(8701)对兔淋巴细胞胞浆内Ca^(2+)浓度的影响。结果表明:8701的各种实验浓度均可显著地促进淋巴细胞内Ca^(2+)浓度增加,在浓度为10^(-4)mol·L^(-1)时,增加细胞内Ca^(2+)浓度的作用显著高于Ca^(2+)载体A23187的作用(P<0.01),提示该药有可能作为淋巴细胞的刺激剂或在高浓度时可能造成淋巴细胞的Ca^(2+)中毒作用。This paper reported the estimation of free calcium concentration in rabbit resting lymphocytes, and the effect of (-) Daurisoline (8701) on the cyto-plasmic free calcium concentration by using fluorescent indicator Fura -2/Am estimating technique. Results were compared with the effect of the Ca2-ionophore A23187. The results indicated that the free calcium concentration of resting lymphocytes was 109. 6nmol · L-1, after 10-5 mol· L-1,10-4.5 mol · L-1,and 10-4 mol · L-1 -)Daurisoline administration, the cyto-plasmic free calcium concentration increased to 178. 8%, 204. 4%and 817. 5% respectively, while same concentration A23187 only led the increase of 164. 6% , 198.4% and 327% respectively. Results suggested that ( - )Daurisoline may be a Ca2- agonist and may induce the cytotoxicity effect on lymphocytes at high concentr ation.

关 键 词：蝙蝠葛苏林碱 淋巴细胞 钙 

分 类 号：R285.5[医药卫生—中药学]