黄皮酰胺抗脂质过氧化及对氧自由基清除作用  

Anti-lipid peroxidation and oxygen free radical scavenging activity of clausenamide

作　　者：林童俊 刘耕陶[1] 李小洁[2] 赵保路[2] 忻文娟[2] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京100050 [2]中国科学院生物物理研究所,北京100080

出　　处：《中国药理学与毒理学杂志》1992年第2期97-102,共6页Chinese Journal of Pharmacology and Toxicology

摘　　要：黄皮酰胺是一种生物碱,可抑制铁-半胱氨酸体系引起的大鼠脑,心,肝和睾丸微粒体脂质过氧化,电子自旋共振法研究表明,黄皮酰可清除由佛波醇豆蔻酸乙酸酸酯(PMA)刺激人多形核白细胞(PML)所产生的氧自由基,自旋探针测氧法结果表明,对PMA刺激PML时的氧消耗无影响,在Fenton反应体系中,对羟自由基的清除率为36.6％,在黄嘌呤-黄嘌呤氧化酶和光照核黄素体系中,对超氧阴离子的清除作用分别为21.2％和16.2％。结果提示黄皮酰胺对氧自由基的直接捕捉作用是其抗脂质过氧化作用的机理之一。Clausenamide (Clau) is an alkaloid. The present investigation demonstrated that Clau inhibited ferrous-cysteine induced lipid peroxidation (malondialdehyde formation) of microsomes from the rat brain, heart, liver and testes. Electron spin resonance (ESR) detected that Clau scavenges oxygen radicals produced by human polymorphonuclear leukocytes (PML) stimulated by phorbol myristate acetate (PMA). However, no inhibitory effect of Clau on oxygen consumption of PML during respiratory burst was measured by spin label oximetry. In Fenton reaction system, 36.6% of OH was scavenged by Clau. It scavenged Oi by 21.2% and 16.2%, in xanthine-xanthine oxidase and UV-irradiation of riboflavin systems, respectively. It may be concluded that Clau exhibits an antioxidant activity via scavenging oxygen free radicals.

关 键 词：脂质过氧化 黄皮酰胺 氧自由基 

分 类 号：R965[医药卫生—药理学]